Difference between revisions of "Team:NCTU Formosa/Safety"

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     <p class="content"Before entering the laboratory, all the members in NCTU_Formosa must receive several training courses and evaluate exams offered by Environmental Protection and Safety Center. The content of the training courses consists of four parts.</p>
 
     <p class="content"Before entering the laboratory, all the members in NCTU_Formosa must receive several training courses and evaluate exams offered by Environmental Protection and Safety Center. The content of the training courses consists of four parts.</p>
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<ul style="list-style-image:none;list-style-type:dics;"
 
     <li class="list">For the basic laboratory safety instruction, we learned the safety level of laboratories and personal protective equipment.</li>
 
     <li class="list">For the basic laboratory safety instruction, we learned the safety level of laboratories and personal protective equipment.</li>
 
     <li class="list">For the laboratory emergency response, we learned of how to react to several emergency situations and certain procedure dealing with specific chemicals.</li>
 
     <li class="list">For the laboratory emergency response, we learned of how to react to several emergency situations and certain procedure dealing with specific chemicals.</li>
 
     <li class="list">For the toxic chemical operation and management, we learned the content of the material safety data sheet(MSDS).</li>
 
     <li class="list">For the toxic chemical operation and management, we learned the content of the material safety data sheet(MSDS).</li>
 
     <li class="list">For biosafety, we learned biological hazard, including levels of biohazard and instructions of dealing with biological trash.</li>
 
     <li class="list">For biosafety, we learned biological hazard, including levels of biohazard and instructions of dealing with biological trash.</li>
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</ul> 
 
     <p class="content">The four training courses contain an evaluating exam. After taking the courses and passing the exam, we all get certificates for each training.</p>
 
     <p class="content">The four training courses contain an evaluating exam. After taking the courses and passing the exam, we all get certificates for each training.</p>
 
      
 
      
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   <ul style="list-style-image:none;list-style-type:decimal;" >
 
   <ul style="list-style-image:none;list-style-type:decimal;" >
 
           <li class="list">Free of <i>E. coli</i>
 
           <li class="list">Free of <i>E. coli</i>
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           <br>The product we spray into the environment should not contain any genetically-modified organisms. So, after we expressed Pantide with <i>E. coli</i>, the LB broth containing <i>E. coli</i> should be first sonicated by the sonicator to let Pantide be soluble in the LB broth. In this step, most of the living <i>E.coli</i> will be eliminated. However, to guarantee the sonicated LB broth is free of <i>E. coli</i>, we put the sonicated LB broth into the pressure-cooker type autoclave for sterilization.
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           <br>&nbsp;&nbsp;The product we spray into the environment should not contain any genetically-modified organisms. So, after we expressed Pantide with <i>E. coli</i>, the LB broth containing <i>E. coli</i> should be first sonicated by the sonicator to let Pantide be soluble in the LB broth. In this step, most of the living <i>E.coli</i> will be eliminated. However, to guarantee the sonicated LB broth is free of <i>E. coli</i>, we put the sonicated LB broth into the pressure-cooker type autoclave for sterilization.
 
           </li>
 
           </li>
 
           <li class="list">Degradation of Pantide
 
           <li class="list">Degradation of Pantide
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           <br>To ensure that Pantide will degrade into amino acids when it is released into the environment, we have done an experiment to prove that Pantide does degrade in the ultraviolet light.(See the <a href="experiment">experiment</a>) Once we have known that Pantide will degrade in the ultraviolet light, we may conclude that Pantide will degrade in the natural light over time. With the degradation modeling simulation(See the <a href="modelling">modelling</a>), we may also apply the degradation rate into the automatic spraying device.
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           <br>&nbsp;&nbsp;To ensure that Pantide will degrade into amino acids when it is released into the environment, we have done an experiment to prove that Pantide does degrade in the ultraviolet light.(See the <a href="experiment">experiment</a>) Once we have known that Pantide will degrade in the ultraviolet light, we may conclude that Pantide will degrade in the natural light over time. With the degradation modeling simulation(See the <a href="modelling">modelling</a>), we may also apply the degradation rate into the automatic spraying device.
 
           </li>
 
           </li>
 
   </ul>
 
   </ul>
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<div>
 
<div>
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     <p class="content-2">For humans, Pantide will not have any negative effect on human health. When the customer gets the agricultural produce from the market, most of the Pantide is thought to have degraded into amino acids and even there is some residue of Pantide on the surface of the product. Pantide is also harmless to humans. The three toxins we choose have been proved to be ineffective to mammals due to the structural difference between the voltage-gated ion channels between mammals and insects. It has been validated through references doing experiments on mice.</p>
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     <p class="content-2"><span style="font-weight:bold;">For humans</b>, Pantide will not have any negative effect on human health. When the customer gets the agricultural produce from the market, most of the Pantide is thought to have degraded into amino acids and even there is some residue of Pantide on the surface of the product. Pantide is also harmless to humans. The three toxins we choose have been proved to be ineffective to mammals due to the structural difference between the voltage-gated ion channels between mammals and insects. It has been validated through references doing experiments on mice.</p>
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     <p class="content-2"><b>For the environment, Pantide is made of amino acids.</b> So it is harmless for the environment. Instead, it can be as the fertilizer. When it is washed into the soil or in water resources, Pantide is ineffective to the microbiomes and the vertebrate in the ecosystem. Furthermore, Pantide is species-specific, so it will not harm the beneficial pollinator, Bees.</p>
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     <p class="content-2"><span style="font-weight:bold;">For the environment, Pantide is made of amino acids.</span> So it is harmless for the environment. Instead, it can be as the fertilizer. When it is washed into the soil or in water resources, Pantide is ineffective to the microbiomes and the vertebrate in the ecosystem. Furthermore, Pantide is species-specific, so it will not harm the beneficial pollinator, Bees.</p>
  
 
</div>   
 
</div>   

Revision as of 15:18, 10 October 2016

Safety

The safety of our project consists of two aspects. One is the laboratory safety; the other is the safety of spraying Pantide into the environment.

Laboratory Safety

    For the basic laboratory safety instruction, we learned the safety level of laboratories and personal protective equipment.
  • For the laboratory emergency response, we learned of how to react to several emergency situations and certain procedure dealing with specific chemicals.
  • For the toxic chemical operation and management, we learned the content of the material safety data sheet(MSDS).
  • For biosafety, we learned biological hazard, including levels of biohazard and instructions of dealing with biological trash.

The four training courses contain an evaluating exam. After taking the courses and passing the exam, we all get certificates for each training.

Safety of Pantide

Processing procedure in the Lab

  • Free of E. coli
      The product we spray into the environment should not contain any genetically-modified organisms. So, after we expressed Pantide with E. coli, the LB broth containing E. coli should be first sonicated by the sonicator to let Pantide be soluble in the LB broth. In this step, most of the living E.coli will be eliminated. However, to guarantee the sonicated LB broth is free of E. coli, we put the sonicated LB broth into the pressure-cooker type autoclave for sterilization.
  • Degradation of Pantide
      To ensure that Pantide will degrade into amino acids when it is released into the environment, we have done an experiment to prove that Pantide does degrade in the ultraviolet light.(See the experiment) Once we have known that Pantide will degrade in the ultraviolet light, we may conclude that Pantide will degrade in the natural light over time. With the degradation modeling simulation(See the modelling), we may also apply the degradation rate into the automatic spraying device.

For humans, Pantide will not have any negative effect on human health. When the customer gets the agricultural produce from the market, most of the Pantide is thought to have degraded into amino acids and even there is some residue of Pantide on the surface of the product. Pantide is also harmless to humans. The three toxins we choose have been proved to be ineffective to mammals due to the structural difference between the voltage-gated ion channels between mammals and insects. It has been validated through references doing experiments on mice.

For the environment, Pantide is made of amino acids. So it is harmless for the environment. Instead, it can be as the fertilizer. When it is washed into the soil or in water resources, Pantide is ineffective to the microbiomes and the vertebrate in the ecosystem. Furthermore, Pantide is species-specific, so it will not harm the beneficial pollinator, Bees.