Difference between revisions of "Team:NCTU Formosa/Composite Part"

Line 114: Line 114:
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);
 
     margin:auto !important;
 
     margin:auto !important;
−
     text-align:center !important1;
+
     text-align:center !important;
 
     color:rgb(0, 206, 255);
 
     color:rgb(0, 206, 255);
−
     margin-top:20px;
+
     margin-top:50px;
 
}
 
}
 
.content{
 
.content{
Line 239: Line 239:
 
<p class="title-1">Composite part</p>
 
<p class="title-1">Composite part</p>
 
<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
 
<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
−
<p class="title"><a href="#" style="biobrick">BBa_456456465</a></p>
+
 
 +
<p class="title"><a href="#" class="biobrick">BBa_456456465</a></p>
 
<p class="content-1">jaksdlfj</p>
 
<p class="content-1">jaksdlfj</p>
 
<p class="content">
 
<p class="content">

Revision as of 14:04, 12 October 2016

Composite part

We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of E. coli we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.

BBa_456456465

jaksdlfj

dsjkfashkfhaskfhsadkjfhsajkfhaksjlfhskjahfk fsajljsdlkfjsakl fsdajlkjsdlfkjsafl; fsdaklfjsaldjf;lsa kldsfjl;sakjfl fdsklajflfal;s afjlkdjfl aksfjlsdafsd fjsdalkfjsl sdlkfjsdlfjal;s klsdajflksjfl lksflksdjflksd lkdsjflksad jkldsjl kjalksfjlsd;faslk kl kl sdlkfjsdlkfjdkjfijelnvkiealf;ieja fjbjiojevnjdkfjduriejdkjfoiewopqjpc