Difference between revisions of "Team:NCTU Formosa/Composite Part"

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<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
 
<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
  
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<p class="title"><a href="#" class="biobrick">Pantide without lectin</a></p>
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<p class="title">Pantide without lectin</p>
 
<p class="content"> Pantide can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders. </p>
 
<p class="content"> Pantide can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders. </p>
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<p class="content-1">BBa_........</p>
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 +
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
  
 
<div>
 
<div>
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Pantide that contains the gene coding for Hv1a linked with His-tag.
 
Pantide that contains the gene coding for Hv1a linked with His-tag.
 
</p>
 
</p>
 +
 
</div>
 
</div>
 
</section>
 
</section>

Revision as of 14:13, 12 October 2016

Composite part

We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of E. coli we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.

Pantide without lectin

Pantide can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders.

BBa_........

T7 promoter+RBS+Hv1a+linker+His-tag+terminator Pantide that contains the gene coding for Hv1a linked with His-tag.