Difference between revisions of "Team:ETH Zurich/Attributions"

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<li> <b> Tsvetan Kardashliev</b> who provided us with sequences and material for directed evolution.
 
<li> <b> Tsvetan Kardashliev</b> who provided us with sequences and material for directed evolution.
 
<li> <b> Alexandra Schulz </b> who supplied us with the Keio reference strain <i>E. coli</i> K-12 BW25113<sup><a href="#cit2" class="cit">2</a></sup>.
 
<li> <b> Alexandra Schulz </b> who supplied us with the Keio reference strain <i>E. coli</i> K-12 BW25113<sup><a href="#cit2" class="cit">2</a></sup>.
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<li><b> Verena Jäggin, Telma Lopes </b> who introduced us to flow cytometry and who optimized its settings for our constructs.
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<li><b> Verena Jäggin and Telma Lopes </b> who introduced us to flow cytometry and who optimized its settings for our constructs.
  
  
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<li><b> Niels Bürckert</b> who gave us our lab and building safety training.
 
<li><b> Niels Bürckert</b> who gave us our lab and building safety training.
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<li><b> The BSSE shop team </b> who kindly assisted us with the purchase of the required materials.
 
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Revision as of 13:14, 15 October 2016

PAVLOV'S COLI

ATTRIBUTIONS

In April 2016 a small group of seven motivated students from the fields of Biology, Biochemistry, Biomedical Engineering, Biotechnology and Computational Sciences started their brainstorming sessions to come up with a real-world issue that could be solved by applying the combined know-how. The ETH iGEM team was responsible for almost all of the tasks, including spending long hours on research, managing our team and lab and designing the genetic circuit. Nevertheless, we were grateful to rely on the expertise and knowledge of our Professors, Advisors and others whose contributions are listed here.

ADVISORS

We were lucky to rely on a team of motivated advisors who were always happy to answer questions, double-check our designs or debug our codes.

  • Claude Lormeau who was always available, even while she was enjoying her holidays.
  • Daniel Gerngross who patiently helped us measure hundreds of FACS samples, even if that meant for him to stay late into the night.
  • Janina Linnik who often came up with elegant ideas to solve modeling problems.
  • Raffaele Altamura who was always able to organize more lab material on short notice when our consumption was higher than expected.

SCIENTIFIC SUPPORT

Within the department as well as outside of it researchers gladly answered our calls for help.

  • Prof. Sven Panke, Prof. Jörg Stelling, Prof. Yaakov Benenson who helped interpreting our results and guided us through important steps of the project.
  • Prof. Martin Jinnek who spared time discussing and evaluating our CRISPR-based system, supplied us with Cpf1 and gRNA's.
  • Prof. Christophe Lacroix who answered many questions regarding the complex intestinal microbiota.
  • Sabine Österle who supplied us with the EcNR1 strain, pCRISPR and pCas9.
  • Sarah Guiziou who sent us the Dual-recombinase-controller plasmid1.
  • Lukas Widmer who introduced us to the cluster.
  • Jan Mikelson who provided us with a useful software tool to access the cluster.
  • Tsvetan Kardashliev who provided us with sequences and material for directed evolution.
  • Alexandra Schulz who supplied us with the Keio reference strain E. coli K-12 BW251132.
  • Verena Jäggin and Telma Lopes who introduced us to flow cytometry and who optimized its settings for our constructs.

GENERAL SUPPORT

  • Niels Bürckert who gave us our lab and building safety training.
  • The BSSE shop team who kindly assisted us with the purchase of the required materials.

REFERENCES

  • [1] Bonnet, Jerome, et al. "Amplifying genetic logic gates." Science 340.6132 (2013): 599-603.
  • [2] Baba, Tomoya, et al. "Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection." Molecular systems biology 2.1 (2006).

Thanks to the sponsors that supported our project: