Difference between revisions of "Team:British Columbia/Project/Bio-Pathways"

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                     <h2>Introduction</h2>
 
                     <h2>Introduction</h2>
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                     <p><i>Escherichia coli</i> is a commonly used synthetic biology platform for biosynthesis of valuable chemicals, attractive due to the availability of genetic manipulation tools and ease of growth and scalability. Past iGEM teams have already built many biosynthesis pathways into E. coli, including the pathway for β-carotene. Our project aimed to utilize engineered <i>E.coli</i> to convert the sugars released from the S-layer degradation of cellulose into value-added chemicals, as a means of reducing the cost of starting materials for these valuable chemicals. We used the previously BioBricked pathway for β-carotene as a proof of concept because β-carotene is coloured, making it easily detectable. β-carotene is naturally found in fruits and vegetables, such as carrots. Coupling the E. coli production system for β-carotene with cheap starting materials, such as plant biomass, suggests that high value chemicals with E. coli biosynthesis pathways could also be produced from cheap starting materials </p>
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                     <p><i>Escherichia coli</i> is a commonly used synthetic biology platform for biosynthesis of valuable chemicals, attractive due to the availability of genetic manipulation tools and ease of growth and scalability. Our project aimed to utilize engineered <i>E.coli</i> to convert the sugars released from the S-layer degradation of cellulose into value-added chemicals, as a means of reducing the cost of starting materials for these valuable chemicals. Past iGEM teams have already built many biosynthesis pathways into <i>E. coli</i>, including the pathway for β-carotene. We used the previously BioBricked pathway for β-carotene as a proof of concept because β-carotene is coloured, making it easily detectable. β-carotene is naturally found in fruits and vegetables, such as carrots. Coupling the <i>E. coli</i> production system for β-carotene with cheap starting materials, such as plant biomass, suggests that high value chemicals with <i>E. coli</i> biosynthesis pathways could also be produced from cheap starting materials </p>
  
 
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Revision as of 17:46, 17 October 2016

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Bio-Pathways

Biosynthetic Pathways

Abstract

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Key Achievements

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Introduction

Escherichia coli is a commonly used synthetic biology platform for biosynthesis of valuable chemicals, attractive due to the availability of genetic manipulation tools and ease of growth and scalability. Our project aimed to utilize engineered E.coli to convert the sugars released from the S-layer degradation of cellulose into value-added chemicals, as a means of reducing the cost of starting materials for these valuable chemicals. Past iGEM teams have already built many biosynthesis pathways into E. coli, including the pathway for β-carotene. We used the previously BioBricked pathway for β-carotene as a proof of concept because β-carotene is coloured, making it easily detectable. β-carotene is naturally found in fruits and vegetables, such as carrots. Coupling the E. coli production system for β-carotene with cheap starting materials, such as plant biomass, suggests that high value chemicals with E. coli biosynthesis pathways could also be produced from cheap starting materials

Design

WE MUST ADD THIS SECTION :)

Methods

Results

Conclusion

References

Check out other parts of our project below!