Difference between revisions of "Team:ShanghaitechChina/Proof"

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{{ShanghaitechChina}}
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<a href="#Abstract">Abstract</a>
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<a href="#Detailed Proof">Detailed Proof</a>
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<a href="#CNanomaterial" style="font-size:14px;margin-left:15px;">Nanomaterial </a>
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<a href="#CBiofilm" style="font-size:14px;margin-left:15px;">Biofilm</a>
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<a href="#CHydrogenase" style="font-size:14px;margin-left:15px;">Hydrogenase </a>
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<a href="#Main points we achieved in our project">Main points</a>
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  <h1 align="center">Abstract</h1>
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Solar Hunter is an artificial hydrogen production system made up of biofilm-anchored nanorods which efficiently convert photons to electrons, and engineered strain expressing [FeFe] Hydrogenase as the acceptor of the electrons to produce hydrogen. The process of making the Solar Hunter entails the testing of each component ’s function separately.
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  <h1 align="center">Detailed Proof</h1>
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a. We, first, need to obtain a suitable nanomaterial that absorbs solar energy and convert it into electrons. The synthesis and characterization data is shown in our webpage, <a href="https://2016.igem.org/Team:ShanghaitechChina/Nanomaterials">nanomaterials</a>
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b. To allow easy recycling and points of aggregation for the small material for a higher efficiency, we utilized biofilm to bind nanomaterials. Please refer to <a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm">Biofilm</a> for the data of the successful construction and the characterization of the binding of nanomaterials to biofilm.<p></p></div>
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c. Finally, high-activity hydrogenase is necessary. We have successfully produced hydrogen many times with our system. Please refer to Hydrogenase for data. Reference <a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen">Hydrogenase <p></p></a>
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  <h1 align="center">Main points we achieved in our project</h1>
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1. Successful synthesis of CdS with desired features.
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2. Confirmed the firm binding of nanomaterials on biofilm composed by CsgA-Histag fused protein.
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Tested the  <a href="http://parts.igem.org/Part:BBa_K2132001">BioBrick BBa_K2132001</a> adhesion to multi-well and micro-beads, and its binding to nano materials within the hydrogen production system.
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3. Transplanted of all the necessary subunits from the gene clusters of [FeFe]-hydrogenase from Clostridium. acetobutylicum onto one single plasmid to allow reliable expression.
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Revision as of 16:31, 18 October 2016

igem2016:ShanghaiTech