Difference between revisions of "Team:ShanghaitechChina/Attributions"

InterLab

We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.

LiuYi BaFang;Zhu Haolong;Wang Qiaoqi

Biofilm Section

An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin.

TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.

Clone of his-CsgA-SpyCatcher-his :Gu Shijie, An Bolin (Graduate Instructor/Advisor)

Clone of his-CsgA-SpyCatcher: Gu Shijie

Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Clone of mcherry-SpyTag: Gu Shijie

Clone of CsgA-SpyCatcher-his: Gu Shijie

Clone of CsgA-SpyCatcher: Gu Shijie

Clone of CsgA: Gu Shijie

Sequencing Confirm: Gu Shijie, An Bolin

An Bolin provided help on Geneious. But the actual alignment was done by Gu Shijie

mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University

ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Colony PCR: Gu Shijie, Qian Lechen

Congo Red Assay(plate): Zheng Weitong, Qian Lechen

Congo Red Assay(solution): Qian Lechen,Zheng Weitong,Zhuang Lei

Expression and purification of mcherry-spycatcher: Zhuang Lei, Qian Lechen, Liu Yi, Zhang Jiaming

Expression and purification of mcherry-spytag: Zhuang Lei, An Bolin, Qian Lehchen

Biofilm culture: Qian Lechen, Zhuanglei, Zheng Weitong

Crystal violet assay: Qian Lechen, Zhang Jiaming

TEM sample preparation: Qian Lechen, Zhang Jiaming，Zhuanglei, Wang Xinyu (Graduate Instructor/Advisor)

TEM: Qian Lechen，Zhang Jiaming, An Bolin, Wang Xinyu

Fluorescence Microscopy: Qian Lechen, An Bolin

Quantum dots(QD) binding test: Qian Lechen,Zhang Jiaming

Different QDs supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Zhang Jiaming, Zhuang Lei

Nanorods binding test: Qian Lechen

Nanorods supply for binding: Zhang Jiaming

mcherry-SpyCatcher and CsgA-SpyTag incubation and sample preparation: Qian Lechen

mcherry-SpyTag and CsgA-SpyCatcher incubation and sample preparation: Qian Lechen

Biofilm expression optimization: Qian Lechen

Confocal fluorescence microscope: Qian Lechen,Li Xiaoming(instructor)

biofilm data analysis and result demostration:Qian Lechen

Biofilm microsphere coculture:Qian Lechen

Reagent and material preparation:Zheng Weitong, Qian Lechen, Gu Shijie, Zhang Jiaming, Zhuanglei

notebook data and protocal collection ：Zheng Weitong

wiki of biofilm: Qian Lechen, Gu Shijie

wiki biofilm picture: Qian Lechen

Human Practice Section

Team Collaboration with iGEM at Jiangsu Normal University: Yi Liu; Fang Ba; Haolong Zhu

Team Collaboration with iGEM at Tsinghua University: Lechen Qian; Haolong Zhu

Public Questionnaire Design: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang

Public Questionnaire Analysis: Haolong Zhu

Interview with Industrial Community: Haolong Zhu; Yi Liu

Interview with Academic Scientists: Haolong Zhu; Lechen Qian; Yi Liu; Jiaming Zhang; Qiaoqi Wang

Interview with Administrative Authority: Haolong Zhu; Yi Liu; Lechen Qian; Jiaming Zhang

Propagation of iGEM (Opening Day for Concordia International School Shanghai): Haolong Zhu; Shijie Gu; Weitong Zheng; Lechen Qian

Wiki of Human Practice: Haolong Zhu

Composition: Haolong Zhu

Photos: Yi Liu; Fang Ba; Haolong Zhu; Lechen Qian

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+<div id="Connection" class="content">
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+<h1 align="center">General</h1>
-                    <h2 class="section-heading">General</h2>
+<h2>iGem is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.
-                    <div class="section-content">
+</h2></div>
- <p style="font-size:12px;margin-left:12px">
+<div class="col-lg-6">
-iGem is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project. </p>
+<center><p style="font-size:18px">
- <p style="font-size:18px">
     Logistics: Chen Yifan, Liu Yi
-   </p>
+   </p></center>
-   <p style="font-size:18px">
+   <center><p style="font-size:18px">
     Abstract: Liu Yi, Ba Fang, Gu Shijie
-   </p>
+   </p></center>
-   <p style="font-size:18px">
+   <center><p style="font-size:18px">
     Parts: Gu Shijie; Chen Yifan
-   </p>
+   </p></center>
-   <p style="font-size:18px">
+   <center><p style="font-size:18px">
     Webpage construction and design: Shen Yifei
-   </p>
+   </p></center>
-                    </div>
+ <center><p style="font-size:18px">
-                </div>
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-                    <div class="section-content2">
-  <p style="font-size:18px">
     wiki Big Picture: Bafang, Gu Shijie
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+<center> <p style="font-size:18px">
     wiki design: Shen Yifei
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+</ul>
+</div>
+<div class="col-lg-6">
+<center> <p style="font-size:18px">
     wiki home background: Ye Qinying
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+ <center> <p style="font-size:18px">
     wiki home video: Gu Shijie, Zhang Jiaming, Ye Qinying, Qian Lechen.
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+<center><p style="font-size:18px">
     wiki logo: Ye Qinying
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+ <center> <p style="font-size:18px">
     Wiki team avatar: JSNU
-   </p>
+   </p></center>
-  <p style="font-size:18px">
+ <center> <p style="font-size:18px">
     Banner&amp;Post: Ba Fang
-   </p>
+   </p></center>
-                    </div>
-                </div>
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+</div></div></div>
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    <div class="content-section-b">
          <div class="container">

Difference between revisions of "Team:ShanghaitechChina/Attributions"

Revision as of 17:25, 18 October 2016

General

InterLab

Hydrogenase Section

Biofilm Section

Nano material Section

Human Practice Section