Abstract

Solar Hunter is an artificial hydrogen production system comprising biofilm-anchored semiconductor nanorods (NRs) which efficiently convert photons to electrons, and engineered strain expressing [FeFe] hydrogenase that can efficiently catalyze Hydrogen production upon receiving the electrons donated by NRs. The success of this integrative hydrogen-producing system relies on robust construction and functional characterization of each part separately. We have proved that we successfully constructed and characterized our parts, as revealed below.

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 <div id="CBiofilm">
-<h2>b. To allow easy recycling of precious semiconductor nanomaterials, we utilized engineered biofilms to anchor nanomaterials via metal coordination chemistry. Please refer to <b><a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm">Biofilm</a> for the successful construction and characterization of engineered biofilms that allow firm binding of nanomaterials.</b></h2><p></p></div>
+<h2>b. To allow easy recycling of precious semiconductor nanomaterials, we utilized engineered biofilms to anchor nanomaterials via metal coordination chemistry. Please refer to <a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm">Biofilm</a> for the successful construction and characterization of engineered biofilms that allow firm binding of nanomaterials.</h2><p></p></div>
 <div id="CHydrogenase">
 <h2>c. Finally, high-activity hydrogenase is necessary for our system. To achieve efficient enzymatic activities, we codon-optimized and constructed the whole hydrogenase gene clusters (from Clostridium Acetobutylicum) by leveraging the multi-expression Acembl System.  Reference <b><a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen">Hydrogenase </b></h2><p></p></a>