Difference between revisions of "Team:British Columbia/Project/Consortia"

Line 107: Line 107:
 
                 <section id="Key Achievements" class="anchor">
 
                 <section id="Key Achievements" class="anchor">
 
                     <h2>Key Achievements </h2>
 
                     <h2>Key Achievements </h2>
−
                    <p></p>
+
<p>
 +
<li> Successfully grew <i>Caulobacter</i> and <i>E.coli</i> in M2 minimal media supplemented with 0.2% glucose.</li>
 +
<li> Co-cultured <i>Caulobacter</i> and <i>E.coli</i> to determine the community dynamics.</li>
 +
<li> Co-cultured cellulase-displaying <i>Caulobacter</i> with β-carotene producing to demonstrate a functional proof of concept of Crescentium project.</i></p>
 +
 
 
                 </section>
 
                 </section>
  
Line 113: Line 117:
 
                     <h2>Introduction</h2>
 
                     <h2>Introduction</h2>
 
                     <p>Microorganisms live in complex microbial communities in the wild, in which individual species with specialized phenotypes interact and cooperate with each other to perform complex metabolic functions. Following nature's examples, there is an increasing trend in using microbial communities for biotechnological application due to their robustness and the ability to perform complex metabolic tasks through the division of labor. Construction of synthetic microbial communities allows to compartmentalize and optimize metabolic functions in different hosts.  
 
                     <p>Microorganisms live in complex microbial communities in the wild, in which individual species with specialized phenotypes interact and cooperate with each other to perform complex metabolic functions. Following nature's examples, there is an increasing trend in using microbial communities for biotechnological application due to their robustness and the ability to perform complex metabolic tasks through the division of labor. Construction of synthetic microbial communities allows to compartmentalize and optimize metabolic functions in different hosts.  
−
The goal of our project is to design a stable, robust microbial community for the production of valuable compounds from lignocellulosic biomass. The metabolic processes are split between biomass-degrading bacteria and the production bacteria, which transforms the degradation products into valuable products. For the first part, we engineered <i>Caulobacter</> displaying functional biomass-transforming enzymes that act on cellulose. For the second part, we engineered <i>E.coli</i> producing β-carotene as a proof of concept. Now we need to confirm that these two bacteria can be co-cultured together to generate a stable consortia for consolidated bioprocessing. </p>
+
The goal of our project is to design a stable, robust microbial community for the production of valuable compounds from lignocellulosic biomass. The metabolic processes are split between biomass-degrading bacteria and the production bacteria, which transforms the degradation products into valuable products. For the first part, we engineered <i>Caulobacter</i> displaying functional biomass-transforming enzymes that act on cellulose. For the second part, we engineered <i>E.coli</i> producing β-carotene as a proof of concept. Now we need to confirm that these two bacteria can be co-cultured together to generate a stable consortia for consolidated bioprocessing. </p>
 
                 </section>
 
                 </section>
 
</div><!--.col-sm-9-->
 
</div><!--.col-sm-9-->

Revision as of 20:56, 18 October 2016

Main CSS Navbar CSS

Sidebar CSS

Consortia

Consortia

Abstract

Key Achievements

  • Successfully grew Caulobacter and E.coli in M2 minimal media supplemented with 0.2% glucose.
  • Co-cultured Caulobacter and E.coli to determine the community dynamics.
  • Co-cultured cellulase-displaying Caulobacter with β-carotene producing to demonstrate a functional proof of concept of Crescentium project.

    • Introduction

    Microorganisms live in complex microbial communities in the wild, in which individual species with specialized phenotypes interact and cooperate with each other to perform complex metabolic functions. Following nature's examples, there is an increasing trend in using microbial communities for biotechnological application due to their robustness and the ability to perform complex metabolic tasks through the division of labor. Construction of synthetic microbial communities allows to compartmentalize and optimize metabolic functions in different hosts. The goal of our project is to design a stable, robust microbial community for the production of valuable compounds from lignocellulosic biomass. The metabolic processes are split between biomass-degrading bacteria and the production bacteria, which transforms the degradation products into valuable products. For the first part, we engineered Caulobacter displaying functional biomass-transforming enzymes that act on cellulose. For the second part, we engineered E.coli producing β-carotene as a proof of concept. Now we need to confirm that these two bacteria can be co-cultured together to generate a stable consortia for consolidated bioprocessing.

    Check out other parts of our project below!