Difference between revisions of "Team:ETH Zurich/NO Release"

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                 <h1>NO RELEASE</h1>
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                 <h1>NITRIC OXIDE RELEASE</h1>
 
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<p>Nitric Oxide release has been a major issue for precise AND gate and NO sensor testing all along the project. Nitric Oxide is a unstable species that degrades extremely quickly in an aqueous medium. It is induced using the DETA NO release mechanism. DETA was chosen because its half like in about 20 h, meaning it produces a slow and regular release, ensuring an almost constant concentration for a long duration.  
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<p>Nitric Oxide (NO) release has been a major issue for precise NO sensor and AND gate testing. NO is a unstable species which degrades quickly in an aqueous medium. It is produced using the DETA/NO release mechanism. DETA was chosen because its half life is about 20 h, meaning it produces a slow and regular release, ensuring an almost constant concentration for a long duration.  
 
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<img src="https://static.igem.org/mediawiki/2016/2/2f/T--ETH_Zurich--NOreleaselowcc.svg">
 
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<p><b>Figure 1: </b>calibration curve for DETA/NO release for DETA concentration range from 10 nM to 500 nM</p>
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<p><b>Figure 1: </b>Calibration curve for DETA/NO release for different DETA concentrations ranging from 10 nM to 500 nM.</p>
 
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<img src="https://static.igem.org/mediawiki/2016/3/33/T--ETH_Zurich--NOreleasehighcc.svg">
 
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<p><b>Figure 2: </b>calibration curve for DETA/NO release for DETA concentration range from 1 mM to 100 mM</p>
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<p><b>Figure 2: </b>Calibration curve for DETA/NO release for DETA concentrations ranging from 1 mM to 100 mM.</p>
 
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Revision as of 21:21, 18 October 2016

PAVLOV'S COLI

NITRIC OXIDE RELEASE

Nitric Oxide (NO) release has been a major issue for precise NO sensor and AND gate testing. NO is a unstable species which degrades quickly in an aqueous medium. It is produced using the DETA/NO release mechanism. DETA was chosen because its half life is about 20 h, meaning it produces a slow and regular release, ensuring an almost constant concentration for a long duration.

REACTION

\begin{align*} DETA&\rightleftharpoons 2 NO\\ 2 NO + O_{2}&\rightleftharpoons 2 NO_{2}\\ \end{align*}

CALIBRATION CURVES

Figure 1: Calibration curve for DETA/NO release for different DETA concentrations ranging from 10 nM to 500 nM.

Figure 2: Calibration curve for DETA/NO release for DETA concentrations ranging from 1 mM to 100 mM.

Thanks to the sponsors that supported our project: