Difference between revisions of "Team:ShanghaitechChina/InterLab"

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We participated in InterLab project this year. We completely finished the work of measuring the expressional level of GFP under different promotor through the method of flow cytometry. With following the standard protocal strictly, the data is reliable.  
 
We participated in InterLab project this year. We completely finished the work of measuring the expressional level of GFP under different promotor through the method of flow cytometry. With following the standard protocal strictly, the data is reliable.  
 
<p></p>
 
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The model of flow cytometry is BD LSRFortessa X-20, collection of the data by the software BD FACSDiva 8.0.1, analysis the data by FlowJo7.6
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The model of flow cytometry is BD LSRFortessa X-20, collection of the data by the software BD FACSDiva 8.0.1, analysis of the data by FlowJo7.6
 
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             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/c/cf/T--ShanghaitechChina--interlab--NC2.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/c/cf/T--ShanghaitechChina--interlab--NC2.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/1/16/T--ShanghaitechChina--interlab--NC3.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/1/16/T--ShanghaitechChina--interlab--NC3.png"  >
<center>we find a low geometric mean in NC for it is set as a control</center>
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<center>We find a low geometric mean in NC for it is set as a control</center>
 
             <h3>For Positive result</h3>
 
             <h3>For Positive result</h3>
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/1/1b/T--ShanghaitechChina--interlab--PC1.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/1/1b/T--ShanghaitechChina--interlab--PC1.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/b/b5/T--ShanghaitechChina--interlab--PC2.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/b/b5/T--ShanghaitechChina--interlab--PC2.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/c/cb/T--ShanghaitechChina--interlab--PC3.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/c/cb/T--ShanghaitechChina--interlab--PC3.png"  >
<center>we find a medium geometric mean compare to NC</center>
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<center>We find a medium geometric mean compare to NC</center>
 
             <h3>For Measurement Kit I</h3>
 
             <h3>For Measurement Kit I</h3>
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/9/9c/T--ShanghaitechChina--interlab--D1-1.png"  >
 
             <img class="pic3x pic3"  src="https://static.igem.org/mediawiki/2016/9/9c/T--ShanghaitechChina--interlab--D1-1.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/a/ab/T--ShanghaitechChina--interlab--D1-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/a/ab/T--ShanghaitechChina--interlab--D1-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/8/85/T--ShanghaitechChina--interlab--D1-3.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/8/85/T--ShanghaitechChina--interlab--D1-3.png"  >
  <center>it is the strongest promotor amang 5. We can observe the green fluorescence by bare eyes.l</center>
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  <center>It is the strongest promotor amang 5. We can observe the green fluorescence by bare eyes.l</center>
 
             <h3>For Measurement Kit II</h3>
 
             <h3>For Measurement Kit II</h3>
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/4/4d/T--ShanghaitechChina--interlab--D2-1.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/4/4d/T--ShanghaitechChina--interlab--D2-1.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/df/T--ShanghaitechChina--interlab--D2-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/df/T--ShanghaitechChina--interlab--D2-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/7/70/T--ShanghaitechChina--interlab--D2-3.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/7/70/T--ShanghaitechChina--interlab--D2-3.png"  >
<center>it had the a general promotor which is a little stronger than PCl</center>
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<center>It had the a general promotor which is a little stronger than PCl</center>
 
             <h3>For Measurement Kit II</h3>
 
             <h3>For Measurement Kit II</h3>
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/d6/T--ShanghaitechChina--interlab--D3-1.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/d6/T--ShanghaitechChina--interlab--D3-1.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/9/9d/T--ShanghaitechChina--interlab--D3-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/9/9d/T--ShanghaitechChina--interlab--D3-2.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/f/f1/T--ShanghaitechChina--interlab--D3-3.png"  >
 
             <img  class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/f/f1/T--ShanghaitechChina--interlab--D3-3.png"  >
<center>it had a weak promoter which is just higher than NC but we can observe it just through a snesitive set of sensor.</center>
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<center>It had a weak promoter which is just higher than NC but we can observe it just through a snesitive set of sensor.</center>
 
       </div>
 
       </div>
 
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Revision as of 02:49, 19 October 2016

igem2016:ShanghaiTech