Difference between revisions of "Team:SUSTech Shenzhen/Parts"
Cockroach627 (Talk | contribs) |
Cockroach627 (Talk | contribs) |
||
| Line 15: | Line 15: | ||
| − | + | === R-GECO coding sequence: === | |
Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence. | Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence. | ||
| − | + | === TetOn regulation system: === | |
Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline. | Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline. | ||
| − | + | === Antibiotics resistance gene: === | |
Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection. | Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection. | ||
| − | + | === Mutated sfGFP coding sequence: === | |
Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site. | Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site. | ||
| Line 35: | Line 35: | ||
= 2. Composite Parts: = | = 2. Composite Parts: = | ||
| − | + | === Chromoproteins: === | |
Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS. | Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS. | ||
| − | + | === sfGFP and mutated sfGFP: === | |
Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence. | Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence. | ||
Revision as of 05:55, 19 October 2016
Parts
Contribution
Contents
This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects. We spared no effort do diversify our BioBricks and we successfully constructed BioBricks of several categories as listed below:
1. Basic Parts:
TRPC5 family:
Sound sensitive channel TRPC5 gene coding sequence with or without mutagenesis on ankyrin repeats region through direct evolution.
R-GECO coding sequence:
Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence.
TetOn regulation system:
Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline.
Antibiotics resistance gene:
Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection.
Mutated sfGFP coding sequence:
Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site.
2. Composite Parts:
Chromoproteins:
Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS.
sfGFP and mutated sfGFP:
Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence.
Parts List
| Type | Name | Description | Length | Characteristics |
|---|---|---|---|---|
| Basic Parts | <partinfo>BBa_K1943012</partinfo> | msfGFP CDS | 717bp | Mutated sfGFP coding sequence without KpnI recognition site |
| <partinfo>BBa_K1943013</partinfo> | Puro CDS | 597bp | Puromycin resistance gene | |
| <partinfo>BBa_K1943014</partinfo> | Bleo CDS | 369bp | Resistance gene to antibiotic phleomycin D1 | |
| <partinfo>BBa_K1943015</partinfo> | Bla CDS | 402bp | Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic | |
| <partinfo>BBa_K1943016</partinfo> | Bla+E2A | 456bp | Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence | |
| <partinfo>BBa_K1943017</partinfo> | Puro+T2A | 651bp | Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence | |
| <partinfo>BBa_K1943018</partinfo> | T2A+Bleo | 429bp | Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence | |
| <partinfo>BBa_K1943019</partinfo> | SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter | 1774bp | Tetracycline-responsive promoter, expression induced by doxycycline | |
| <partinfo>BBa_K1943020</partinfo> | mR-GECO CDS | 1257bp | Mutated R-GECO CDS without 2 PstI in original sequence | |
| <partinfo>BBa_K1943021</partinfo> | TRPC5 | 2928bp | original TRPC5 CDS | |
| <partinfo>BBa_K1943022</partinfo> | TRPC5 | 2928bp | mutated TRPC5 CDS -7 | |
| <partinfo>BBa_K1943023</partinfo> | TRPC5 | 2928bp | mutated TRPC5 CDS -12 | |
| <partinfo>BBa_K1943024</partinfo> | TRPC5 | 2928bp | mutated TRPC5 CDS -15 | |
| <partinfo>BBa_K1943025</partinfo> | TRPC5 | 2928bp | mutated TRPC5 CDS -18 | |
| <partinfo>BBa_K1943026</partinfo> | TRPC5 | 2928bp | mutated TRPC5 CDS -20 | |
| Composite Parts | <partinfo>BBa_K1943000</partinfo> | J23100+ B0034+ fwYellow+ B0010+ B0012 | 912bp | Yellow chromoprotein reporter system (Strong Promoter, Strong RBS) |
| <partinfo>BBa_K1943001</partinfo> | J23110+ B0031+ fwYellow+ B0010+ B0012 | 914bp | Yellow chromoprotein reporter system (medium Promoter , weak RBS) | |
| <partinfo>BBa_K1943002</partinfo> | J23100+ B0034+ gfasPurple + B0010+ B0012 | 867bp | Purple chromoprotein reporter system (Strong Promoter, Strong RBS) | |
| <partinfo>BBa_K1943003</partinfo> | J23110+ B0031+ gfasPurplee + B0010+ B0012 | 869bp | Purple chromoprotein reporter system (medium Promoter , weak RBS) | |
| <partinfo>BBa_K1943004</partinfo> | J23100+ B0034+ eforRed + B0010+ B0012 | 879bp | Red chromoprotein reporter system (Strong Promoter, Strong RBS) | |
| <partinfo>BBa_K1943005</partinfo> | J23110+ B0031+ eforRed + B0010+ B0012 | 881bp | Red chromoprotein reporter system (medium Promoter , weak RBS) | |
| <partinfo>BBa_K1943006</partinfo> | J23100+ B0034+ spisPink + B0010+ B0012 | 876bp | Pink chromoprotein reporter system (Strong Promoter, Strong RBS) | |
| <partinfo>BBa_K1943007</partinfo> | J23110+ B0031+ spisPink + B0010+ B0012 | 878bp | Pink chromoprotein reporter system (medium Promoter , weak RBS) | |
| <partinfo>BBa_K1943008</partinfo> | J23116+B0034+sfGFP | 778bp | Strong green fluorescence | |
| <partinfo>BBa_K1943009</partinfo> | J23116+B0034+sfGFP+ B0010+B0012 | 918bp | Strong green fluorescence | |
| <partinfo>BBa_K1943010</partinfo> | J23116+B0034+msfGFP | 781bp | Strong green fluorescence without KpnI recognition site in CDS | |
| <partinfo>BBa_K1943011</partinfo> | J23116+B0034+msfGFP+ B0010+B0012 | 915bp | Strong green fluorescence without KpnI recognition site in CDS |
