Difference between revisions of "Team:Stony Brook/Parts"
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<h3 style="font-family: Georgia;"> HIV Antigen Expression System (K1908002) </h3> | <h3 style="font-family: Georgia;"> HIV Antigen Expression System (K1908002) </h3> | ||
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| + | <h1 style="font-family: Georgia;"> BioBrick Improvements </h1> | ||
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| + | <h3 style="font-family: Georgia;"> fwYellow-amilCP Osmolarity Color Toggle (K1908000) </h3> | ||
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| + | This system is intended to be a blue-yellow color toggle that expresses fwYellow (K1033910) and amilCP (K592009) dependent on the activation of the OmpC promoter at varied ambient osmolarities. A negative feedback loop inhibits production of fwYellow (regulated by pLac) when the OmpC promoter is active and amilCP/LacI is being produced. Growth in high-osmolarity media is expected to give a blue color while growth in low-osmolarity media is expected to produce a yellow color. Further characterization of the construct is necessary to understand the mathematical relationship between protein expression levels at varied ambient osmolarities. | ||
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Revision as of 06:17, 19 October 2016
New BioBricks
Cripto-1 Expression System (K1908001)
This part is a semi-composite part that was synthesized by IDT which holds a Saccharomyces cerevisiae Kozak sequence, a coding sequence for the Homo sapien membrane protein Cripto-1, and an ADH1 Terminator region. The coding sequence was codon-optimized for expression in yeast using the proprietary codon optimization program on the Integrated DNA Technologies website. Our design was intended to induce a strong expression of Cripto-1 in yeast to test for membrane localization. We hope that our contribution of a eukaryotic receptor protein to the iGEM Registry is found to be useful and beneficial.
HIV Antigen Expression System (K1908002)
BioBrick Improvements
fwYellow-amilCP Osmolarity Color Toggle (K1908000)
This system is intended to be a blue-yellow color toggle that expresses fwYellow (K1033910) and amilCP (K592009) dependent on the activation of the OmpC promoter at varied ambient osmolarities. A negative feedback loop inhibits production of fwYellow (regulated by pLac) when the OmpC promoter is active and amilCP/LacI is being produced. Growth in high-osmolarity media is expected to give a blue color while growth in low-osmolarity media is expected to produce a yellow color. Further characterization of the construct is necessary to understand the mathematical relationship between protein expression levels at varied ambient osmolarities.
Parts
| Part Name | Part Description |
|---|---|
| BBa_K592009 | Amil CP, Blue chromaprotein |
| BB_J23119 | Constitutive Promoter |
| BBa_B0015 | Terminator |
| BBa_E0040 | GFP |
| BBa_K1908000 | BY-Toggle |
| BBa_K1908001 | Cripto-1 Coding Sequence | BBa_K1908002 | TBA |
