Difference between revisions of "Team:ShanghaitechChina/Demonstrate"
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<h1 align="center">Demonstration of Solar Hunter in a Nutshell</h1> | <h1 align="center">Demonstration of Solar Hunter in a Nutshell</h1> | ||
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| − | Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. | + | Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. Furthermore, the intrinsic adherence of biofilms towards various interfaces allows us to grow biofilms on easy-separation micro-beads, therefore facilitating recyclable usage of the biofilm-anchored NRs and endowing this whole system with recyclability. Notably, our hydrogen production has shown great stability compared to some precursors using hydrogenase. Such system can also be adapted to other energy-oriented applications by utilizing engineered new strains with a diverse spectrum of enzymes or metabolic pathways. In this section, we show the successful hydrogen production data with all the components. For the thorough tour of hydrogen production assays we did, please refer to Integrative Bio-hydrogen System (https://2016.igem.org/Team:ShanghaitechChina/IBS). <p></p> |
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In the activity assay of the hydrogenase in producing hydrogen, the system goes through three periods of “light-on and light-off”. The result (see below) shows the stability of the system and the reversible catalytic activity of the hydrogenase of the reaction, 2H+ + 2e- ⇿ H2 .<p></p><h3 id="AInstrument">(2) Instrument</h3> | In the activity assay of the hydrogenase in producing hydrogen, the system goes through three periods of “light-on and light-off”. The result (see below) shows the stability of the system and the reversible catalytic activity of the hydrogenase of the reaction, 2H+ + 2e- ⇿ H2 .<p></p><h3 id="AInstrument">(2) Instrument</h3> | ||
| − | <center><img src="https://static.igem.org/mediawiki/2016/ | + | <div class="col-lg-6"> |
| − | <p style="text-align:center"><b>Figure | + | <center><img src="https://static.igem.org/mediawiki/2016/6/60/T--ShanghaitechChina--chanqingzhuangzhizuizhong.png" style="width:72%"></center></div> |
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| + | <center><img src="https://static.igem.org/mediawiki/2016/0/06/T--ShanghaitechChina--Hydrogenase--chanqingzhuangzhixijie.png" style="width:72%"></center> | ||
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| + | <center><p style="text-align:center"><b>Figure 1</b> Apparatus of the hydrogen production assay.</p></center> | ||
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It contains (1) an anaerobic reaction container which is a transparent circular cuvette that allows light to go through; (2) a light source in our hydrogen production assay acting as a substitute for the real sun. (We chose a high-power white LED light, set 28cm away from the reaction container for a even distribution of photons); (3) a hydrogen electrode linked to its inner sensor inserted into the reaction container to measure the realtime concentration of hydrogen; (4) a date hub; (5) a computer connected to the hub to record the data and generate the curve of concentration variation within a period of time. <p></p> | It contains (1) an anaerobic reaction container which is a transparent circular cuvette that allows light to go through; (2) a light source in our hydrogen production assay acting as a substitute for the real sun. (We chose a high-power white LED light, set 28cm away from the reaction container for a even distribution of photons); (3) a hydrogen electrode linked to its inner sensor inserted into the reaction container to measure the realtime concentration of hydrogen; (4) a date hub; (5) a computer connected to the hub to record the data and generate the curve of concentration variation within a period of time. <p></p> | ||
Revision as of 10:31, 19 October 2016
