General

Logistics

Yifan Chen, Yi Liu

Abstract

Yi Liu, Fang Ba, Shijie Gu

Parts

Shijie Gu, Yifan Chen

Webpage

Yifei Shen

wiki Big Picture

Lechen Qian, Fang Ba

wiki design

Yifei Shen

wiki home background: Qinying Ye

wiki home video: Shijie Gu, Jiaming Zhang, Qinying Ye, Lechen Qian.

wiki logo: Qinying Ye

Wiki team avatar: JSNU

Banner&Post: Fang Ba

Special Notes:iGEM is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.

InterLab

Yi Liu,BaFang,Zhu Haolong,Qiaoqi Wang

Special Notes:We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.

Hydrogenase Section

Clone Design: Yilin Sun, Yifan Chen, Yan Nie, Yi Liu

Sequencing Confirm: Yi Liu, Yifan Chen

Clone hydrogenase gene clusters:,Yi Liu, Fang Ba, Yifan Chen, Haonan Yang

Cre recombination & Result Demonstration: Bafang, Yi Liu

Hydrogen Assay: Yi Liu, Fang Ba, Qixi Mi, Zetao Ding

Preparation of component cell : Yi Liu, Yifan Chen

HydA Induction, Purification and WB Demonstration: Zhu Haolong, Qiaoqi Wang

Wiki of Hydrogenase : Yi Liu, Shijie Gu, Fang Ba

Special Notes:In this part Yan Nie provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Yan Nie’s Lab, SIAIS. The help of Yan Nie was involved in the the beginning, but the principle is that all of the experiments are done by ourselves. Yilin Sun provided us with the tutorials of Snapgene and primer design.The sequencing is provided by Tsingke Sequencing and Jie Li Sequencing.Mi Qixi is a PI from School of Physical Science and Technology. Ding Zetao is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.

Biofilm Section

Clone of a library of CsgA fused protein and sequencing confirm :Shijie Gu, An Bolin (Graduate Instructor/Advisor)

Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Clone of mcherry-SpyTag: Shijie Gu

mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University

ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Colony PCR: Shijie Gu, Lechen Qian

Biofilm Characterization: Lechen Qian, Jiaming Zhang

Biofilm culture: Lechen Qian, Zhuanglei, Weitong Zheng

Different nanomaterials supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Jiaming Zhang, Lei Zhuang

Biofilm expression optimization: Lechen Qian

biofilm data analysis and result demostration: Lechen Qian

notebook data and protocal collection ：Weitong Zheng

wiki of biofilm: Lechen Qian, Shijie Gu

wiki biofilm picture: Lechen Qian

Special Notes:An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin. TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.

Nano material Section

Quantum dots, nanorods and ligand synthesis: Jiaming Zhang, Lei Zhuang, Kang Wang

Quantum dot and nanorods characterization:Jiaming Zhang

wiki of Quantum Dots: Jiaming Zhang, Lei Zhuang

Special Notes:Pu Jiahua and Wang Xinyu are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and UV-Vis spectrometer characterization. Wang helped in the synthesis of HS-NTA synthesis, TEM characterization and PL spectrometer characterization.

Human Practice Section

Team Collaborations: Yi Liu; Haolong Zhu; Lechen Qian; Fang Ba

Public Questionnaire: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang

Social Interviews: Haolong Zhu; Yi Liu; Jiaming Zhang; Lechen Qian; Yifan Chen

Propagation of iGEM: Haolong Zhu; Shijie Gu; Lechen Qian; Weitong Zheng

Wiki of Human Practice: Haolong Zhu; Fang Ba

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