Biofilm Section
Clone of a library of CsgA fused protein and sequencing confirm
Shijie Gu, An Bolin (Graduate Instructor/Advisor)
Clone of CsgA-his
A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
Clone of mcherry-SpyTag
Shijie Gu
mCherry-SpyCatcher strain
A gift of Zhong Chao Lab at ShanghaiTech University
CsgA-KO stra
in
A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
Colony PCR
Shijie Gu, Lechen Qian
Biofilm Characterization
Lechen Qian, Jiaming Zhang
Biofilm culture
Lechen Qian, Zhuanglei, Weitong Zheng
Different nanomaterials supply for characterization
Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Jiaming Zhang, Lei Zhuang
Biofilm expression optimization
Lechen Qian
biofilm data analysis and result demostration
Lechen Qian
notebook data and protocal collection
Weitong Zheng
wiki of biofilm
Lechen Qian, Shijie Gu
wiki biofilm picture
Lechen Qian
Special Notes:An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin. TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.
