Difference between revisions of "Team:BIT-China"

Line 527: Line 527:
 
                 <h3 class="header-xsmall">Our P-SLACKiller</h3>
 
                 <h3 class="header-xsmall">Our P-SLACKiller</h3>
 
                 <div class="bottom-title margin-bottom-10">
 
                 <div class="bottom-title margin-bottom-10">
                     Plasmids are losing, and the production efficiency is decreasing. Let’s equip the bacteria with a plasmid-sensing logically adjustable killer (P-SLACKiller) to kill slackers with less plasmids among bacteria!                </div>
+
                     Plasmids are losing, and the production efficiency is decreasing. Let’s equip the bacteria with a plasmid-sensing logically adjustable cell killer (P-SLACKiller) to kill slackers with less plasmids among bacteria!                </div>
 
             </div>
 
             </div>
  

Revision as of 14:55, 19 October 2016

overtop
overview1 In the procedure of large-scale industrial
production, due to the random distribution of
plasmids in the process of bacteria proliferation
and division, the bacteria containing less
plasmids will gradually become the
“slackers”.
overview2 Generally, we aim to avoid these undesired
bacteria become the dominant population.
There is no efficient way to prevent the birth of
plasmid-free bacteria and the applied ways are
only limited to the laboratory-use.
overview3
What about equipping the bacteria with a
plasmid-sensing logically adjustable cell killer
(P-SLACKiller)?
Here is killer’s basic rule:
Kill the slackers (Plasmid numbers are above a
threshold);
Save the workers (Plasmid numbers are below
the threshold).
overview4
Surprise! We can guarantee the microbial
population structure stay optimal
now!
overview5

Our team divide the project into 4 parts as below.
Why not click and have a look?

home-basic

Our P-SLACKiller

Plasmids are losing, and the production efficiency is decreasing. Let’s equip the bacteria with a plasmid-sensing logically adjustable cell killer (P-SLACKiller) to kill slackers with less plasmids among bacteria!
home-basic

Threshold Device

In what situation can the bacteria be judged as slackers? There must have a threshold concerning plasmid numbers. Let’s find it out and adjust it accordingly.
home-basic

Killer Device

For the slackers, they should be able to turn on the self-killing switch. We tried toxin proteins and double strand break (DSB) to produce the lethal effect.
home-basic

Optimization

The executor killer should be stably maintained, so we integrated killer device into the genome. Moreover, we mutated promoters to adjust the threshold.