Project Design

Metabolic pathway engineering was used to modify Lactobacillus johnsonii for increased fructose metabolism. Those modifications aim to increase fructose uptake, while reducing glucose consumption, because both carbohydrates display the main energy sources of L. johnsonii ATCC33200. To search for targets in both metabolic pathways the patric database was used. L. johnsonii’s genome was documented and analysed in course of the Human Microbiome Project in 2009. Glucose and fructose metabolism in wild type L. johnsonii (FIGURE 1) differs from our engineered bacterium in following points:

To down regulate the glucose metabolism, a gene knockout via CRISPR/Cas9 system was planned. Carbohydrates are taken up into the cell via transporters of the ABC-superfamily, getting phosphorylated in this process, but for glucose no specific transporter was annotated. Therefore the two main enzymes for glucose-6-phosphate metabolism were targeted: Glucose-6-phosphate-isomerase is part of glycolysis, converting glucose-6-phosphate to fructose-6-phosphate and reverse, as well as glucose-6-phosphate-dehydrogenase which converts glucose-6-phosphate to 6-phospho-D-glucono-1,5-lactone, being the starting point for the pentose phosphate pathway. These two knockouts should result in an accumulation of glucose-6-phosphate which in turn causes decreased glucose uptake by product inhibition of glucose ingesting transporters. On the other hand, fructose metabolism should be enhanced by upregulation of endogenous enzymes: Fructose enters the cell as monosaccharide or, in conjunction with glucose, as sucrose. Both carbohydrates are taken up by substrate specific PTS transporters and get phosphorylated in this process. Therefore both transporters were identified as possible targets for pathway enhancement.

Besides, phosphofructokinase was chosen for elevated fructose turnover: This enzyme catalyzes the reaction of fructose-6-phosphate to fructose-1,6-bisphosphat preventing fructose-6-phosphate accumulation after increased uptake. Enhancing this enzyme’s activity should result in higher ATP production via glycolysis using mainly fructose and sucrose as substrates. Enhancement of fructose and sucrose metabolism should be achieved by introducing the three endogenous proteins via the lactobacillus shuttle vector pNZ124Tue. The hypothetic results of the metabolic engineering and its effects are summed up in FIGURE 2. After we picked out possible knock-out and overexpression targets by educated guessing a systems biology approach was developed to simulate the proliferative behaviour of our modified lactobacillus. To get more information about our modeling approach please klick here.

In human, hereditary fructose intolerance is caused by accumulation of fructose-1-phosphate. To simulate this cellular problem in a model organism, a ketohexokinase was codon optimized for expression in Saccharomyces cerevisiae. This experiment is based on a paper by Donaldson et al from 1993REFERNCE. Yeast cannot metabolize fructose-1-phosphate causing it to be toxic for the cells and inhibiting it’s growth. For this reason no yeast enzyme produces fructose-1-phosphate. So the idea was to introduce a ketohexokinase into yeast leading to the production and accumulation of fructose-1-phosphate in fructose containing environment. Hence, if there is any fructose consuming organism the modified yeast cells should grow better. The ketohexokinase designed for this project is based on the ketohexokinase of Rattus norvegicus, originally used for the experiments in Donaldson’s publication.