Difference between revisions of "Team:Stony Brook/Safety2"

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<li> We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms </li>
 
<li> We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms </li>
 
<ol>
 
<ol>
−
<li>Nitrile glvoes</li>
+
<li>Nitrile gloves</li>
 +
<li>Closed-toe shoes and long pants</li>
 +
<li>UV-protective glasses/goggles for DNA gel analysis</li>
 +
</ol>
 
<li> Gloves were changed after working with said organisms to prevent cross contamination </li>
 
<li> Gloves were changed after working with said organisms to prevent cross contamination </li>
 
<li> No organisms were brought out of the lab at any time </li>
 
<li> No organisms were brought out of the lab at any time </li>

Revision as of 19:17, 19 October 2016

Safety

BSL-1 Organisms

  • Stony Brook iGEM worked with E. coli and S. cerevisiae
  • We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms
    1. Nitrile gloves
    2. Closed-toe shoes and long pants
    3. UV-protective glasses/goggles for DNA gel analysis
  • Gloves were changed after working with said organisms to prevent cross contamination
  • No organisms were brought out of the lab at any time

Handling of chemicals

  • All chemicals, such as Ethidium Bromide and Glacial Acetic Acid were kept in specified areas and were carried in secondary containers as well to minimize risk of spilling

Non-Pathogenic Parts

  • Constructs ordered from IDT or otherwise synthesized were made from coding sequences that code for non-pathogenic or infectious molecules