Difference between revisions of "Team:Stony Brook/Safety2"
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<h3> BSL-1 Organisms </h3> | <h3> BSL-1 Organisms </h3> | ||
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<li> Stony Brook iGEM worked with <i>E. coli</i> and <i>S. cerevisiae</i> </li> | <li> Stony Brook iGEM worked with <i>E. coli</i> and <i>S. cerevisiae</i> </li> | ||
<li> We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms </li> | <li> We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms </li> | ||
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<li>Nitrile gloves</li> | <li>Nitrile gloves</li> | ||
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<li>UV-protective glasses/goggles for DNA gel analysis</li> | <li>UV-protective glasses/goggles for DNA gel analysis</li> | ||
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<li> Gloves were changed after working with said organisms to prevent cross contamination </li> | <li> Gloves were changed after working with said organisms to prevent cross contamination </li> | ||
<li> No organisms were brought out of the lab at any time </li> | <li> No organisms were brought out of the lab at any time </li> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
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Revision as of 19:41, 19 October 2016
Safety
BSL-1 Organisms
- Stony Brook iGEM worked with E. coli and S. cerevisiae
- We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms
- Nitrile gloves
- Closed-toe shoes and long pants
- UV-protective glasses/goggles for DNA gel analysis
- Gloves were changed after working with said organisms to prevent cross contamination
- No organisms were brought out of the lab at any time
Handling of chemicals
- All chemicals, such as Ethidium Bromide and Glacial Acetic Acid were kept in specified areas and were carried in secondary containers as well to minimize risk of spilling
Non-Pathogenic Parts
- Constructs ordered from IDT or otherwise synthesized were made from coding sequences that code for non-pathogenic or infectious molecules
