Difference between revisions of "Team:ShanghaitechChina/Biofilm"

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<p style="text-align:center"><b>Fig 10.</b> Quantum Dots Templating Assay on His-CsgA-SpyCatcher-Histag Biofilm.</p>
 
<p style="text-align:center"><b>Fig 10.</b> Quantum Dots Templating Assay on His-CsgA-SpyCatcher-Histag Biofilm.</p>
 
</center></div><div class="col-lg-7">
 
</center></div><div class="col-lg-7">
After applying the same steps as introduced above, the bottom of left well show a large area of bright fluorescence, manifesting His-CsgA-SpyCatcher-Histag mutant secreted biofilms under the control of inducer and Histags on it is not blocked by SpyCatcher protein. What is more, it is firmly attached with inorganic materials (i.e.quantum dots) through the ligand. From this assay, we assure that the SpyCatcher will not impose negative effect on the binding between nanomaterial and biofilm. The picture was snapped by ChemiDoc MP, BioRad, false colored.</p><p></p></div>
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After applying the same steps as introduced above, the bottom of left well show a large area of bright fluorescence, manifesting His-CsgA-SpyCatcher-Histag mutant secreted biofilms under the control of inducer and Histags on it is not blocked by SpyCatcher protein. What is more, it is firmly attached with inorganic materials (i.e.quantum dots) through the ligand. From this assay, we assure that the SpyCatcher will not impose negative effect on the binding between nanomaterial and biofilm. The picture was snapped by ChemiDoc MP, BioRad, false colored.</p><p></p></div> </div>
 
<b  class="tc">His-CsgA-SpyCatcher</b><p></p><div class="col-lg-12"><div class="col-lg-7">
 
<b  class="tc">His-CsgA-SpyCatcher</b><p></p><div class="col-lg-12"><div class="col-lg-7">
 
Using the same approach, we also conducted binding assay of His-CsgA-SpyCatcher with QDs to characterize the expression of biofilm and  the visual result shows vividly that His-CsgA-SpyCatcher can bind successfully with the QDs with the existence of inducer aTc, which shows the functional similarity in CsgA-Histag. The picture was snapped by BioRad ChemiDoc MP, false colored.<p></p></div>
 
Using the same approach, we also conducted binding assay of His-CsgA-SpyCatcher with QDs to characterize the expression of biofilm and  the visual result shows vividly that His-CsgA-SpyCatcher can bind successfully with the QDs with the existence of inducer aTc, which shows the functional similarity in CsgA-Histag. The picture was snapped by BioRad ChemiDoc MP, false colored.<p></p></div>
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</center>
 
</center>
 
<p style="text-align:center"><b>Fig 11.</b> Quantum dots templating assay on His-CsgA-SpyCatcher biofilm.</p>
 
<p style="text-align:center"><b>Fig 11.</b> Quantum dots templating assay on His-CsgA-SpyCatcher biofilm.</p>
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<h4><b>3. TEM: visualization of binding test</b></h4>
 
<h4><b>3. TEM: visualization of binding test</b></h4>
  

Revision as of 21:32, 19 October 2016

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