Difference between revisions of "Team:EPFL/Safety"

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                        <h2 class="lead">Safety</h2>
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                        <h3>GENERAL</h3>
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                            Working in the lab means that  we have  safety guidelines! First of all, lab coat and gloves are mandatory along with safety glasses where needed. We start every experiment cleaning  our workbench with ethanol and end that same experiment doing exactly the same, preventing growth of contaminants. Furthermore, we separated the benches used for E. coli and Yeast to avoid contamination. When working near the flames we avoided using gloves. All bacterial and yeast cultures were neutralized with bleach before disposal and put in the biowaste. Posttreatment of the waste was done by the home institute facility and according to the legislation in our country.
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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                        <h3>SPECIAL MEASURES</h3>
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<h5>Safe Project Design</h5>
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                            We are working with dCas9, a catalytically dead version of CRISPR-Cas9, which unlike its active form isn’t able to cut DNA sequences but it only binds to them depending on the guide RNA (gRNA). Therefore the danger from off-targets is already much lower. That did not stop us from checking for any binding sequences for gRNAs in the yeast and human genome (even though we do not intend to use our gates in humans) to add another layer of security. The risk in this could be unwanted inhibition/activation of some of the genes in our own genome! Luckily, we were able to design and obtain gRNAs with no such human-binding capabilities!
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 22:32, 19 October 2016

iGEM EPFL 2016

Safety

GENERAL

Working in the lab means that we have safety guidelines! First of all, lab coat and gloves are mandatory along with safety glasses where needed. We start every experiment cleaning our workbench with ethanol and end that same experiment doing exactly the same, preventing growth of contaminants. Furthermore, we separated the benches used for E. coli and Yeast to avoid contamination. When working near the flames we avoided using gloves. All bacterial and yeast cultures were neutralized with bleach before disposal and put in the biowaste. Posttreatment of the waste was done by the home institute facility and according to the legislation in our country.

SPECIAL MEASURES

We are working with dCas9, a catalytically dead version of CRISPR-Cas9, which unlike its active form isn’t able to cut DNA sequences but it only binds to them depending on the guide RNA (gRNA). Therefore the danger from off-targets is already much lower. That did not stop us from checking for any binding sequences for gRNAs in the yeast and human genome (even though we do not intend to use our gates in humans) to add another layer of security. The risk in this could be unwanted inhibition/activation of some of the genes in our own genome! Luckily, we were able to design and obtain gRNAs with no such human-binding capabilities!