Difference between revisions of "Team:NTU-Singapore/Basic Part"

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{{NTU-Singapore}}
 
{{NTU-Singapore}}
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">basic part special prize</a>. </p>
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    <title>NTU-Singapore</title>
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<a href="https://2016.igem.org/Team:NTU-Singapore" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x fa-home"></i> <span class="navbar-name"> NTU-SG</span> </a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Proof" style="height: 49px;"><i class="fa fa-2x  fa-bar-chart" style="float: left;"></i><span style="width: 96px;
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<a href="https://2016.igem.org/Team:NTU-Singapore/Basic_Part"><i class="fa fa-fw fa-2x fa-gear"></i><span style="left: 8px;"class="navbar-name"> Basic Parts</span> </a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Composite_Part" style="height: 45px"><i class="fa fa-fw fa-2x fa-gavel" style="float: left;"></i><span style="float: left; width: 96px; left: 13px;" class="navbar-name"> Composite Part</span> </a>
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left: 13px;" class="navbar-name"> CRISPR<span style="text-transform: lowercase;">y</span> Collection</span> </a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/HP/Gold"><i class="fa fa-fw fa-2x fa-envelope"></i><span class="navbar-name"> Gold</span> </a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Engagement"><i class="fa fa-fw fa-2x fa-heartbeat"></i><span class="navbar-name"> Engagement</span></a>
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<p>
 
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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                <div class="jumbotron content-page" style="font-family: 'Just Me Again Down Here', cursive; padding-top:150px; height: 340px;">
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<h1 style="font-size: 70px;"><span style="color: #0089A7; ">Basic</span> Parts</h1>
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<div class="page-header">
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<h2>Truncated dCas9</h2>
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<div class="well">
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<p>BBa_K2130000 ∆RuvCIII-2 ∆HNH SP-dCas9</p>
  
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<p>This tuncated version of dCas9 is the smallest dCas9 that works the best. The ∆RuvCIII-2 truncation has been rationally predicted by comparison of crystal structures between two states of Cas9.</p>
  
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<div style="margin: auto; width: 900px;">
  
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<img class="content-img" src="https://static.igem.org/mediawiki/2016/0/0b/T--NTU-Singapore--ruvc3.jpg" alt="" style="width:900px; height:379px; box-shadow: none;">
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<p style="font-size:18px; text-align: center;">Conformations of RuvCIII in state A, before NTS cleavage and state B, after NTS cleavage</p>
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<p>It is able to retain ~70% of binding strength with 15% of the protein is deleted.</p>
  
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<div style="margin: auto; width: 706px;">
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<img class="content-img" src="https://static.igem.org/mediawiki/2016/a/a2/T--NTU-Singapore--ruvc3delresult.png" alt="" style="box-shadow: none; width:706px; height:367px;">
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<p style="font-size:18px; text-align: center;">Activation of a zsGreen reporter gene using the various RuvCIII truncations</p>
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Revision as of 00:43, 20 October 2016

NTU-Singapore

Basic Parts

Truncated dCas9

BBa_K2130000 ∆RuvCIII-2 ∆HNH SP-dCas9

This tuncated version of dCas9 is the smallest dCas9 that works the best. The ∆RuvCIII-2 truncation has been rationally predicted by comparison of crystal structures between two states of Cas9.

Conformations of RuvCIII in state A, before NTS cleavage and state B, after NTS cleavage

It is able to retain ~70% of binding strength with 15% of the protein is deleted.

Activation of a zsGreen reporter gene using the various RuvCIII truncations