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Revision as of 02:38, 20 October 2016

Interlab Study

Our InterLab study on the standardization of GFP fluorescence was an overall success and provided us with a fun activity with which to take our minds from the other projects we were working on. We were able to obtain very clean results across the range of our fluorescence and absorbance tests and hope that our data can help to contribute to iGEM's quest to more fully characterize the widely used GFP marker.



Absorbance values for replaicates across the 6-hours data collection process. It can be seen that device 1 experienced a low level of growth across the time period compared to each of the alternative devices and controls.

As expected, we noticed a low level of fluorescnece in our readings for the negative control across all time points in the experiment. It was also seen that device 3 demonstrated a low level of GFP production in E. Coli.

This bar graph provides average FI/Abs600 readings across all controls and devices. Standard deviations were calculated using an averaging of data from the two replicates for each control and device and are represented by the error whiskers on the bars.