Difference between revisions of "Team:UIUC Illinois/Composite Part"

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       <td><a href="http://parts.igem.org/Part:BBa_K1987030">BBa_K1987030</a></td>
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       <td><a href="http://parts.igem.org/Part:BBa_K1987000">BBa_K1987000</a></td>
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       <td>On->off promoter</td>
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       <td>GFP with BsaI sites for promoter insertion</td>
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       <td>Regulatory</td>
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       <td>Composite</td>
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       <td>This promoter can be inserted upstream of any gene in order to provide timed gene expression. This promoter is active during the early growth phase of e. coli, but becomes less active later on, leading to an "on -> off" expression pattern.</td>
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       <td>This testing construct consists of a degradation-tagged GFP orf preceded by the lac promoter and RBS. The promoter and RBS are flanked by BsaI sites, allowing for different promoter+RBS combinations to be easily swapped into the construct. The strength and expression profile of the promoter/RBS combo can then be easily measured.</td>
 
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Revision as of 02:38, 20 October 2016

UIUC_Illinois iGEM 2016

Composite Part

Our team developed the following composite part to aid us in testing our basic parts:

Part number Name Type Brief Summary
BBa_K1987000 GFP with BsaI sites for promoter insertion Composite This testing construct consists of a degradation-tagged GFP orf preceded by the lac promoter and RBS. The promoter and RBS are flanked by BsaI sites, allowing for different promoter+RBS combinations to be easily swapped into the construct. The strength and expression profile of the promoter/RBS combo can then be easily measured.

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