Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W7"

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</table>
 
</table>
 
</ul>
 
</ul>
 +
 +
</div>  - *THIS DIV CONTAINER CLOSES EVERYTHING ABOVE THIS. PLEASE REMOVE THIS ONE LATER ON WHEN YOU GET RID OF THE TEMPLATES.
 +
 +
<h4> Multiple Digests Template: </h4>
 +
 +
<div class="column half_size">
 +
<div align="center">
 +
<h5> Lane 1: PIggyBac - 121.2 ng/ul </h5>
 +
</div>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental ul </th>
 +
<th> Control ul </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O </td>
 +
<td> 35.75</td>
 +
<td> 36.75 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 5 </td>
 +
<td> 5 </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 8.25 </td>
 +
<td> 8.25 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
 +
 +
<div class="column half_size">
 +
<div align="center">
 +
<h5> Lane 2: PIggyBac 1:10 - 423.7 ng/ul </h5>
 +
</div>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental ul </th>
 +
<th> Control ul </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O </td>
 +
<td> 41,6</td>
 +
<td> 42.6 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 5 </td>
 +
<td> 5 </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 2.36 </td>
 +
<td> 2.36 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
 +
 +
<div class="column full_size">
 +
<br>
 +
</div>
 +
 +
<div class="column half_size">
 +
<div align="center">
 +
<h5> Lane 4: Ryan - 446.4 ng/ul </h5>
 +
</div>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental ul </th>
 +
<th> Control ul </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O </td>
 +
<td> 41.8</td>
 +
<td> 42.8 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 5 </td>
 +
<td> 5 </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 2.24 </td>
 +
<td> 2.24 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
 +
 +
<div class="column half_size">
 +
<div align="center">
 +
<h5> Lane 7: Ryan 3 - 272.6 ng/ul </h5>
 +
</div>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental ul </th>
 +
<th> Control ul </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O </td>
 +
<td> 40.3</td>
 +
<td> 41.3  </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 5 </td>
 +
<td> 5 </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 3.67 </td>
 +
<td> 3.67 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
  
  

Revision as of 07:30, 5 August 2016

MENU ▤

Week 7: 8/8 - 8/14

Week 7: 8/8 - 8/14

8/8

PCR Table Template:
    Contents ul
    Phire 25
    H2O 23.2
    50 uM F Primer 0.5
    50 uM R Primer 0.5
    95.1ug/ul Construct 0.8

PCR Settings Template:

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 60
    Denaturation 98 25
    Annealing 66 20
    Extension 72 45
    Final Extension 72 60

Nanodrop Template:

    Content Concentration (ng/ul) 260/280
    Construct 14.6 1.85
    Vector 14.6 1.80

Ligation Template

    Content ul
    10X T4 Buffer 2
    Vector 4 (58.4 ng)
    Construct 1.55 (22.69 ng)
    H2O 11.45
    Ligase 1
- *THIS DIV CONTAINER CLOSES EVERYTHING ABOVE THIS. PLEASE REMOVE THIS ONE LATER ON WHEN YOU GET RID OF THE TEMPLATES.

Multiple Digests Template:

Lane 1: PIggyBac - 121.2 ng/ul
    Content Experimental ul Control ul
    H2O 35.75 36.75
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 8.25 8.25
Lane 2: PIggyBac 1:10 - 423.7 ng/ul
    Content Experimental ul Control ul
    H2O 41,6 42.6
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 2.36 2.36

Lane 4: Ryan - 446.4 ng/ul
    Content Experimental ul Control ul
    H2O 41.8 42.8
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 2.24 2.24
Lane 7: Ryan 3 - 272.6 ng/ul
    Content Experimental ul Control ul
    H2O 40.3 41.3
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 3.67 3.67

8/9

8/10

8/11

8/12

8/13

8/14