Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W7"

Line 66: Line 66:
 
<td> Initial Denaturation </td>
 
<td> Initial Denaturation </td>
 
<td> 98 </td>
 
<td> 98 </td>
−
<td> 60 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>  
 
<tr>  
 
<td> Denaturation </td>
 
<td> Denaturation </td>
 
<td> 98 </td>
 
<td> 98 </td>
−
<td> 25 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Annealing </td>
 
<td> Annealing </td>
−
<td> 66 </td>
+
<td> # </td>
−
<td> 20 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Extension </td>
 
<td> Extension </td>
 
<td> 72 </td>
 
<td> 72 </td>
−
<td> 45 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Final Extension </td>
 
<td> Final Extension </td>
 
<td> 72 </td>
 
<td> 72 </td>
−
<td> 60 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
</table>
 
</table>
Line 101: Line 101:
 
<tr>
 
<tr>
 
<td> Construct </td>
 
<td> Construct </td>
−
<td> 14.6 </td>
+
<td> # </td>
−
<td> 1.85 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Vector </td>
 
<td> Vector </td>
−
<td> 14.6 </td>
+
<td> # </td>
−
<td> 1.80 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
Line 122: Line 122:
 
<tr>
 
<tr>
 
<td> 10X T4 Buffer </td>
 
<td> 10X T4 Buffer </td>
−
<td> 2 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Vector </td>
 
<td> Vector </td>
−
<td> 4 (58.4 ng) </td>
+
<td> # (# ng) </td>
 
</tr>
 
</tr>
 
<td> Construct </td>
 
<td> Construct </td>
−
<td> 1.55 (22.69 ng) </td>
+
<td> # (# ng) </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> H<sub>2</sub>O</td>
 
<td> H<sub>2</sub>O</td>
−
<td> 11.45 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Ligase </td>
 
<td> Ligase </td>
−
<td> 1</td>
+
<td> #</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
Line 152: Line 152:
 
<div class="column half_size">
 
<div class="column half_size">
 
<div align="center">
 
<div align="center">
−
<h5> Lane 1: PIggyBac - 121.2 ng/ul </h5>
+
<h5> This </h5>
 
</div>
 
</div>
 
<ul>
 
<ul>
Line 163: Line 163:
 
<tr>  
 
<tr>  
 
<td> H<sub>2</sub>O </td>
 
<td> H<sub>2</sub>O </td>
−
<td> 35.75</td>
+
<td> #</td>
−
<td> 36.75 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Cutsmart </td>
 
<td> Cutsmart </td>
−
<td> 5 </td>
+
<td> # </td>
−
<td> 5 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> EcoRI-HF </td>
 
<td> EcoRI-HF </td>
−
<td> 0.5 </td>
+
<td> # </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> XbaI </td>
 
<td> XbaI </td>
−
<td> 0.5 </td>
+
<td># </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> DNA </td>
 
<td> DNA </td>
−
<td> 8.25 </td>
+
<td> # </td>
−
<td> 8.25 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
</table>
 
</table>
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<div class="column half_size">
 
<div class="column half_size">
 
<div align="center">
 
<div align="center">
−
<h5> Lane 2: PIggyBac 1:10 - 423.7 ng/ul </h5>
+
<h5> A </h5>
 
</div>
 
</div>
 
<ul>
 
<ul>
Line 203: Line 203:
 
<tr>  
 
<tr>  
 
<td> H<sub>2</sub>O </td>
 
<td> H<sub>2</sub>O </td>
−
<td> 41,6</td>
+
<td> #</td>
−
<td> 42.6 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Cutsmart </td>
 
<td> Cutsmart </td>
−
<td> 5 </td>
+
<td> # </td>
−
<td> 5 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> EcoRI-HF </td>
 
<td> EcoRI-HF </td>
−
<td> 0.5 </td>
+
<td> # </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> XbaI </td>
 
<td> XbaI </td>
−
<td> 0.5 </td>
+
<td># </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> DNA </td>
 
<td> DNA </td>
−
<td> 2.36 </td>
+
<td> # </td>
−
<td> 2.36 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
</table>
 
</table>
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<div class="column half_size">
 
<div class="column half_size">
 
<div align="center">
 
<div align="center">
−
<h5> Lane 4: Ryan - 446.4 ng/ul </h5>
+
<h5> Is </h5>
 
</div>
 
</div>
 
<ul>
 
<ul>
Line 247: Line 247:
 
<tr>  
 
<tr>  
 
<td> H<sub>2</sub>O </td>
 
<td> H<sub>2</sub>O </td>
−
<td> 41.8</td>
+
<td> #</td>
−
<td> 42.8 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Cutsmart </td>
 
<td> Cutsmart </td>
−
<td> 5 </td>
+
<td> # </td>
−
<td> 5 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> EcoRI-HF </td>
 
<td> EcoRI-HF </td>
−
<td> 0.5 </td>
+
<td> # </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> XbaI </td>
 
<td> XbaI </td>
−
<td> 0.5 </td>
+
<td># </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> DNA </td>
 
<td> DNA </td>
−
<td> 2.24 </td>
+
<td> # </td>
−
<td> 2.24 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
</table>
 
</table>
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<div class="column half_size">
 
<div class="column half_size">
 
<div align="center">
 
<div align="center">
−
<h5> Lane 7: Ryan 3 - 272.6 ng/ul </h5>
+
<h5> Thing </h5>
 
</div>
 
</div>
 
<ul>
 
<ul>
Line 287: Line 287:
 
<tr>  
 
<tr>  
 
<td> H<sub>2</sub>O </td>
 
<td> H<sub>2</sub>O </td>
−
<td> 40.3</td>
+
<td> #</td>
−
<td> 41.3  </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> Cutsmart </td>
 
<td> Cutsmart </td>
−
<td> 5 </td>
+
<td> # </td>
−
<td> 5 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> EcoRI-HF </td>
 
<td> EcoRI-HF </td>
−
<td> 0.5 </td>
+
<td> # </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> XbaI </td>
 
<td> XbaI </td>
−
<td> 0.5 </td>
+
<td># </td>
 
<td> X </td>
 
<td> X </td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td> DNA </td>
 
<td> DNA </td>
−
<td> 3.67 </td>
+
<td> # </td>
−
<td> 3.67 </td>
+
<td> # </td>
 
</tr>
 
</tr>
 
</table>
 
</table>

Revision as of 07:50, 5 August 2016

MENU ▤

Week 7: 8/8 - 8/14

I left you some of the commonly used templates down there under 8/8. Just fill them in as needed. I'll only really have wifi for a few hours on two days between Saturday and Thursday. If you need anything, message me on Facebook and I'll get back to you as soon as I can.

With the warmest love and regards,

~JOSHUA

Week 7: 8/8 - 8/14

8/8

PCR Table Template:
    Contents ul
    Phire #
    H2O #
    50 uM F Primer #
    50 uM R Primer #
    #ug/ul Construct #

PCR Settings Template:

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 #
    Denaturation 98 #
    Annealing # #
    Extension 72 #
    Final Extension 72 #

Nanodrop Template:

    Content Concentration (ng/ul) 260/280
    Construct # #
    Vector # #

Ligation Template

    Content ul
    10X T4 Buffer #
    Vector # (# ng)
    Construct # (# ng)
    H2O #
    Ligase #

Multiple Digests Template:

This
    Content Experimental ul Control ul
    H2O # #
    Cutsmart # #
    EcoRI-HF # X
    XbaI # X
    DNA # #
A
    Content Experimental ul Control ul
    H2O # #
    Cutsmart # #
    EcoRI-HF # X
    XbaI # X
    DNA # #

Is
    Content Experimental ul Control ul
    H2O # #
    Cutsmart # #
    EcoRI-HF # X
    XbaI # X
    DNA # #
Thing
    Content Experimental ul Control ul
    H2O # #
    Cutsmart # #
    EcoRI-HF # X
    XbaI # X
    DNA # #

8/9

8/10

8/11

8/12

8/13

8/14