Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W9"

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<h4> PCR Settings: 25 Cycles
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<h4> PCR Settings: 25 Cycles</h4>
 
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Revision as of 21:50, 23 August 2016

Week 9: 8/22 - 8/28

Week 9: 8/22 - 8/28



8/22

PCR of new IDT Shipments

AmilCP
    Contents ul
    Q5 MasterMix 12.5
    H2O 9
    10 uM F Primer 1.25
    10 uM R Primer 1.25
    Template 1
IDT #1
    Contents ul
    Q5 MasterMix 12.5
    H2O 6.5
    10 uM F Primer 2.5
    10 uM R Primer 2.5
    Template 1

IDT #2
    Contents ul
    Q5 MasterMix 12.5
    H2O 6.5
    10 uM F Primer 2.5
    10 uM R Primer 2.5
    Template 1

PCR Settings: 25 Cycles

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 30
    Denaturation 98 10
    Annealing 65 30
    Extension 72 120
    Final Extension 72 120


8/23



8/24