Team:ShanghaitechChina/Biofilm

igem2016:ShanghaiTech

Biofilm Introduction

We envision to establish the Solar Hunter system on E.Coli’s biofilm. Biofilms are ubiquitous since they can be found both in human and some extreme environments. They can be formed on inert surfaces of devices and equipment, which will be hard to clean and cause dysfunction of the device.
However, we view it through different lenses to transform this ill impact into merits. Biofilm can substantially increase the resistance of bacteria to adverse conditions like acid or oxidative stress and form a stable and balanced system. This traits can elevate its adaptability to application to industry for they don’t need to be meticulously taken care of and capable to withstand harsh conditions. Therefore, it’ll be a good practice to reduce the production cost. What’s more, biofilm can automatically grow by static adherence , which facilitate regeneration and recycling in mass production in industry. Startlingly, biofilms can also serve as a synthetic nonconductive biological platform for self-assembling function materials. The amyloid protein CsgA , which is the dominant component in E.Coli, can be programmed to append small peptide domain and successfully secreted with biological functions. For that reason, biofilm became our best candidate to engineer and would be equipped with the function we want. Here, we conceive the semiconductor-enzyme system based on E.Coli’s biofilm, whose subunits are engineered respectively with PolyHistidine tags and SpyTag and SpyCatcher system from FbaB protein to provide binding sites for quantum dots and enzymes. Our ultimate goal is to harness this bio-abiotic hybrid system to efficiently convert solar energy into alternative energy or other high value-added industrial products.

Motivation

An article (abbreviated to ART) is a word (prefix or suffix) that is used alongside a noun to indicate the type of reference being made by the noun. Articles specify grammatical definiteness of the noun, in some languages extending to volume or numerical scope. The articles in the English language are the and a/an, and (in certain contexts) some. "An" and "a" are modern forms of the Old English "an", which in Anglian dialects was the number "one" (compare "on", in Saxon dialects) and survived into Modern Scots as the number "owan". Both "on" (respelled "one" by the Normans) and "an" survived into Modern English, with "one" used as the number and "an" ("a", before nouns that begin with a consonant sound) as an indefinite article.In many languages, articles are a special part of speech, which cannot easily be combined with other parts of speech. In English, articles are frequently

Motivation

An article (abbreviated to ART) is a word (prefix or suffix) that is used alongside a noun to indicate the type of reference being made by the noun. Articles specify grammatical definiteness of the noun, in some languages extending to volume or numerical scope. The articles in the English language are the and a/an, and (in certain contexts) some. "An" and "a" are modern forms of the Old English "an", which in Anglian dialects was the number "one" (compare "on", in Saxon dialects) and survived into Modern Scots as the number "owan". Both "on" (respelled "one" by the Normans) and "an" survived into Modern English, with "one" used as the number and "an" ("a", before nouns that begin with a consonant sound) as an indefinite article.In many languages, articles are a special part of speech, which cannot easily be combined with other parts of speech. In English, articles are frequently

Motivation

An article (abbreviated to ART) is a word (prefix or suffix) that is used alongside a noun to indicate the type of reference being made by the noun. Articles specify grammatical definiteness of the noun, in some languages extending to volume or numerical scope. The articles in the English language are the and a/an, and (in certain contexts) some. "An" and "a" are modern forms of the Old English "an", which in Anglian dialects was the number "one" (compare "on", in Saxon dialects) and survived into Modern Scots as the number "owan". Both "on" (respelled "one" by the Normans) and "an" survived into Modern English, with "one" used as the number and "an" ("a", before nouns that begin with a consonant sound) as an indefinite article.In many languages, articles are a special part of speech, which cannot easily be combined with other parts of speech. In English, articles are frequently

Motivation

An article (abbreviated to ART) is a word (prefix or suffix) that is used alongside a noun to indicate the type of reference being made by the noun. Articles specify grammatical definiteness of the noun, in some languages extending to volume or numerical scope. The articles in the English language are the and a/an, and (in certain contexts) some. "An" and "a" are modern forms of the Old English "an", which in Anglian dialects was the number "one" (compare "on", in Saxon dialects) and survived into Modern Scots as the number "owan". Both "on" (respelled "one" by the Normans) and "an" survived into Modern English, with "one" used as the number and "an" ("a", before nouns that begin with a consonant sound) as an indefinite article.In many languages, articles are a special part of speech, which cannot easily be combined with other parts of speech. In English, articles are frequently