Team:ETH Zurich/Experiments
Wiki under construction
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration
| Component | 50 µl Reaction | |
| Nuclease-free water | to 50 µl | |
| 5X Phusion HF | 10 µl | |
| 10 mM dNTPs | 1 µl | |
| 10 µM Forward Primer | 2.5 µl | |
| 10 µM Reverse Primer | 2.5 µl | |
| Template DNA | variable | < 250 ng |
| DMSO (optional) | (1.5 µl, up to 3%) | |
| Phusion DNA Polymerase |
0.5 µl |
