Team:Kyoto/Experiments
Protocols
Ligation
Ligation was performed according to Ligation high Ver.2’s protocol. 1. unfreeze competent cells on hands. put them on ice. 2. add DNA sample (1~20ul) to competent cells. leave them on ice for 30 minutes 3. keep them in heating block 45 seconds, then on ice for 30 minutes. 4. add 40~200ul SOC liquid culture medium, then incubate it under shaking culture for 1hour 37℃. 5. seed it onto LB+antibiotics +/- culture. incubate it for 24 hour at 37℃.
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