Team:Kyoto/Experiments

Protocols

Ligation

Ligation was performed according to Ligation high Ver.2’s protocol.

1. unfreeze competent cells on hands. put them on ice.
2. add DNA sample (1~20ul) to competent cells. leave them on ice for 30 minutes
3. keep them in heating block 45 seconds, then on ice for 30 minutes.
4. add 40~200ul SOC liquid culture medium, then incubate it under shaking culture for 1hour 37℃.
5. seed it onto LB+antibiotics +/- culture. incubate it for 24 hour at 37℃.

PCR

Ligation was performed according to Ligation high Ver.2’s protocol.

1. unfreeze competent cells on hands. put them on ice.
2. add DNA sample (1~20ul) to competent cells. leave them on ice for 30 minutes
3. keep them in heating block 45 seconds, then on ice for 30 minutes.
4. add 40~200ul SOC liquid culture medium, then incubate it under shaking culture for 1hour 37℃.
5. seed it onto LB+antibiotics +/- culture. incubate it for 24 hour at 37℃.

header 1

header 2

header 3

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist

header 1

header 2

header 3

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist

header 1

header 2

header 3

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

texttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttexttext

  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist
  • listlistlistlistlist