Logistics: Chen Yifan, Liu Yi
Abstract: Liu Yi, Ba Fang, Gu Shijie
Parts: Gu Shijie; Chen Yifan
Webpage construction and design: Shen Yifei
Team:ShanghaitechChina/Attributions
General
wiki Big Picture: Bafang, Gu Shijie
wiki design: Shen Yifei
wiki home background: Ye Qinying
wiki home video: Gu Shijie, Zhang Jiaming, Ye Qinying, Qian Lechen.
wiki logo: Ye Qinying
Wiki team avatar: JSNU
Banner&Post: Ba Fang
InterLab
We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.
LiuYi BaFang;Zhu Haolong;Wang Qiaoqi
Hydrogenase Section
Clone Design: Sun Yilin, Chen Yifan, Nie Yan, LiuYi
In this part Nie Yan provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Nie Yan’s Lab, SIAIS. The help of Nie Yan was involved in the the beginning, but the principle is that all of the experiments are done by ourselves.
Sun Yilin provided us with the tutorials of Snapgene and primer design.
Sequencing Confirm: LiuYi, Chen Yifan
The sequencing is provided by Tsingke Sequencing and Jie Li Sequencing
Clone of histag-TEV-hydA-SpyCatcher into pACE:Yang Haonan, Liu Yi, Ba Fang
Clone of histag-TEV-hydA-SpyTag into pACE: Yang Haonan, Liu Yi, Ba Fang
Clone of hydE into pDC: Chen Yifan, Liu Yi, Ba Fang
Clone of hydF into pDK: Ba Fang, Liu Yi
Clone of hydG into pDS: Liu Yi, Ba Fang
Cre recombination & Result Demonstration: Bafang, LiuYi
Hydrogen Assay: LiuYi, Ba Fang, Mi Qixi, Ding Zetao
Mi Qixi is a PI from School of Physical Science and Technology. Ding Zetao is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.
Preparation of PirLC/DH5a/BL21(DE3) chemistry component cell : LiuYi
Preparation of DH5a electronic component cell : Chen Yifan
HydA Purification & WB Demonstration: Zhu Haolong, Wang Qiaoqi
Wiki of Hydrogenase : Liu Yi, Gu Shijie, Ba Fang
Biofilm Section
An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin.
TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.
Clone of his-CsgA-SpyCatcher-his :Gu Shijie, An Bolin (Graduate Instructor/Advisor)
Clone of his-CsgA-SpyCatcher: Gu Shijie
Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
Clone of mcherry-SpyTag: Gu Shijie
Clone of CsgA-SpyCatcher-his: Gu Shijie
Clone of CsgA-SpyCatcher: Gu Shijie
Clone of CsgA: Gu Shijie
Sequencing Confirm: Gu Shijie, An Bolin
An Bolin provided help on Geneious. But the actual alignment was done by Gu Shijie
mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University
ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
Colony PCR: Gu Shijie, Qian Lechen
Congo Red Assay(plate): Zheng Weitong, Qian Lechen
Congo Red Assay(solution): Qian Lechen,Zheng Weitong,Zhuang Lei
Expression and purification of mcherry-spycatcher: Zhuang Lei, Qian Lechen, Liu Yi, Zhang Jiaming
Expression and purification of mcherry-spytag: Zhuang Lei, An Bolin, Qian Lehchen
Biofilm culture: Qian Lechen, Zhuanglei, Zheng Weitong
Crystal violet assay: Qian Lechen, Zhang Jiaming
TEM sample preparation: Qian Lechen, Zhang Jiaming，Zhuanglei, Wang Xinyu (Graduate Instructor/Advisor)
TEM: Qian Lechen，Zhang Jiaming, An Bolin, Wang Xinyu
Fluorescence Microscopy: Qian Lechen, An Bolin
Quantum dots(QD) binding test: Qian Lechen,Zhang Jiaming
Different QDs supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Zhang Jiaming, Zhuang Lei
Nanorods binding test: Qian Lechen
Nanorods supply for binding: Zhang Jiaming
mcherry-SpyCatcher and CsgA-SpyTag incubation and sample preparation: Qian Lechen
mcherry-SpyTag and CsgA-SpyCatcher incubation and sample preparation: Qian Lechen
Biofilm expression optimization: Qian Lechen
Confocal fluorescence microscope: Qian Lechen,Li Xiaoming(instructor)
biofilm data analysis and result demostration:Qian Lechen
Biofilm microsphere coculture:Qian Lechen
Reagent and material preparation:Zheng Weitong, Qian Lechen, Gu Shijie, Zhang Jiaming, Zhuanglei
notebook data and protocal collection ：Zheng Weitong
wiki of biofilm: Qian Lechen, Gu Shijie
wiki biofilm picture: Qian Lechen
Nano material Section
Pu Jiahua and Wang Xinyu are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and UV-Vis spectrometer characterization. Wang helped in the synthesis of HS-NTA synthesis, TEM characterization and PL spectrometer characterization.
Quantum dots, nanorods and ligand synthesis: Zhang Jiaming, Zhuang Lei, Wang Kang
Quantum dot and nanorods characterization:Zhang Jiaming
wiki of Quantum Dots: Zhang Jiaming, Zhuang lei
Human Practice Section
Team Collaboration with iGEM at Jiangsu Normal University: Yi Liu; Fang Ba; Haolong Zhu
Team Collaboration with iGEM at Tsinghua University: Lechen Qian; Haolong Zhu
Public Questionnaire Design: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang
Public Questionnaire Analysis: Haolong Zhu
Interview with Industrial Community: Haolong Zhu; Yi Liu
Interview with Academic Scientists: Haolong Zhu; Lechen Qian; Yi Liu; Jiaming Zhang; Qiaoqi Wang
Interview with Administrative Authority: Haolong Zhu; Yi Liu; Lechen Qian; Jiaming Zhang
Propagation of iGEM (Opening Day for Concordia International School Shanghai): Haolong Zhu; Shijie Gu; Weitong Zheng; Lechen Qian
Composition: Haolong Zhu
Photos: Yi Liu; Fang Ba; Haolong Zhu; Lechen Qian
