Team:ShanghaitechChina/Parts

igem2016:ShanghaiTech

Introduction

For our project, we decided to submit seven BioBricks to the iGEM Registry in the pSB1C3 plasmid backbone. Our submitted parts can be divided into two separate collections (Names as Parts collection one and Part Collection two). Parts collection one is associated with Engineered biofilm subunit CsgA, while Parts collection two is relevant to construction of hydrogenase gene clusters.

All of the constructs are protein-coding parts have been confirmed with gene sequences, demonstrated with expected functionalities, and thus it would be easy to handle for other users.

Specifically, in order to make hydrogenase expression more productive, we optimized the gene sequences of 5 hydrogenase parts with the help of OptimumGeneTM (GenScript). We changed the codon usage bias in E. Coli by upgrading the CAI (Codon Adaptation Index) from a low level (around 0.30) to 0.97

Characterizations of the parts are included on the webpage of the parts section, but for more information about the submitted parts in real use, please go to project page

title

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