Team:ETH Zurich/Detector Module

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• REPORTER MODULE
• Overview
• Goals
• Model
• Results
• References

REPORTER MODULE

OVERVIEW

Figure 1: Schematic of the reporter of our circuit.

The reporter is the component of the circuit that enables readout of the stored information. The state of the switch is displayed by two different fluorescent proteins: sfGFP is expressed by DNA that has been switched, while DNA that didn't switch expresses mNectarine.

In order to implement multiplexing, the reporter proteins are expressed only when they are induced by the same candidate marker that triggered the switch.

• GOALS

• MODEL

• RESULTS

GOALS

• Assist the design of the reporter.

• Characterize the reporter proteins.

MODEL

Our reporter system consist in two fluorenscent proteins that show the state of the switch. After induction, a switched plasmid expresses GFP, while a plasmid in it's original state expresses mNectarine.

Figure 2: Biological implementation of the integrase reporter. The figure shows both the switched and non switched state. Expression of the reporter proteins is repressed by default and induced in presence of the candidate marker.

The following section describes the species and reactions involved:

REACTIONS

\begin{align*} 1) & P_{mNect} & \rightarrow P_{mNect} + mRNA_{mNect} \\ 2) & P_{sfGFP} & \rightarrow P_{sfGFP} + mRNA_{sfGFP} \\ 3) & mRNA_{mNect} & \rightarrow mRNA_{mNect} + mNect \\ 4) & mRNA_{sfGFP} & \rightarrow mRNA_{sfGFP} + sfGFP \\ 5) & mRNA_{mNect} & \rightarrow \\ 6) & mRNA_{sfGFP} & \rightarrow \\ 7) & mNect & \rightarrow \\ 8) & sfGFP & \rightarrow \\ \end{align*}

SPECIES

Name	Description
PmNect	Non switched promoter, facing the mNect gene.
PsfGFP	Switched promoter, facing the sfGFP gene.
mRNAmNect	mRNA of the mNectarine protein.
mRNAsfGFP	mRNA of the sfGFP protein.
mNect	mNectarine fluorescent protein.
sfGFP	Superfolder GFP protein.

ODEs:

\begin{align*} P_{mNect} & \rightarrow P_{mNect} + mRNA_{mNect} \\ P_{sfGFP} & \rightarrow P_{sfGFP} + mRNA_{sfGFP} \\ mRNA_{mNect} & \rightarrow mRNA_{mNect} + mNect \\ mRNA_{sfGFP} & \rightarrow mRNA_{sfGFP} + sfGFP \\ mRNA_{mNect} & \rightarrow \\ mRNA_{sfGFP} & \rightarrow \\ mNect & \rightarrow \\ sfGFP & \rightarrow \\ \end{align*}

PARAMETERS

Name	Description
PmNect	Non switched promoter, facing the mNect gene.
PsfGFP	Switched promoter, facing the sfGFP gene.
mRNAmNect	mRNA of the mNectarine protein.
mRNAsfGFP	mRNA of the sfGFP protein.
mNect	mNectarine fluorescent protein.
sfGFP	Superfolder GFP protein.

RESULTS

REFERENCES

• [1] -

Thanks to the sponsors that supported our project:

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