Thanks for the help and guidance!
We are very gratefull that we have these super helpful labmates that are willing to spend time teaching us! They are the key to our success! :)
Advisors
Asst. Prof Tan Meng How
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Dr. Teh Min Yan
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Dr. Yuan Ming
Dr. Li Jia
Liu Kaiwen Ivy
Adventurers
Ooi Kean Hean
Student leader of the team. Designed the wiki, Cas9 models and exhibition items. In charge on Project Truncation, analysis of crystal structure of Sp-dCas9 for determination of RuvCIII, REC2 and REC1 truncations, Gibson cloning of truncation regions, maintanence of HEK293FT cells and preparation for transfection, did flow cytometry and analysis of the results.
Yong Jing Yen
Responsible for submission of parts and experiments involved in collaboration. Designed Cas9 models, poster and exhibition items. Cloned constructs for Project Evaluation and carried out T7E assay, transfection of HEK293 cells, FACS preparation and genomic PCR
Liu Zhehui
Designed the brochure for our exhibition. Designed the wiki. Photographer of our team. Helped out in Project Truncation, i.e. Gibson cloning of truncation regions, preparation of HEK293FT cells for transfection, flow cytometry, and derived truncation regions in the PI domain. Cloned constructs for Project Evaluation and carried out T7E assay and genomic PCR
Li Fanxiang
Carried out all the assays involved in Project Evolution. Photographer of our team. Drew all the fantastic picture of us!
Mu Huanwen
Carried out all the assays involved in Project Evolution and testing the mutant Cas9 in HEK 293 cells
Zhang Junyi
Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, preparation of PCR products for deep sequencing.
Shaun Lim Wen Zheng
>Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, preparation of PCR products for deep sequencing.
Ming Jie
Helped out during our exhibition
Bui Thuy Thien
Helped out during our exhibition
Jenndy
Helped out during our exhibition