Team:NTU-Singapore/Attributions

NTU-Singapore

Thanks for the help and guidance!

We are very gratefull that we have these super helpful labmates that are willing to spend time teaching us! They are the key to our success! :)

Advisors

Asst. Prof Tan Meng How

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Dr. Teh Min Yan

Devised the method for the evolution of Cas9 and mentored Fanxiang and Huanwen for the Evolution project.

Dr. Yuan Ming

Liu Kaiwen Ivy

Adventurers

Ooi Kean Hean

Student leader of the team. Designed the wiki, Cas9 models and exhibition items. In charge on Project Truncation, analysis of crystal structure of Sp-dCas9 for determination of RuvCIII, REC2 and REC1 truncations, Gibson cloning of truncation regions, maintanence of HEK293FT cells and preparation for transfection, did flow cytometry and analysis of the results.

Yong Jing Yen

Responsible for submission of parts and experiments involved in collaboration. Designed Cas9 models, poster and exhibition items. Cloned constructs for Project Evaluation and carried out T7E assay, transfection of HEK293 cells, FACS preparation and genomic PCR

Liu Zhehui

Designed the brochure for our exhibition. Designed the wiki. Photographer of our team. Helped out in Project Truncation, i.e. Gibson cloning of truncation regions, preparation of HEK293FT cells for transfection, flow cytometry, and derived truncation regions in the PI domain. Cloned constructs for Project Evaluation and carried out T7E assay and genomic PCR

Li Fanxiang

Carried out all the assays involved in Project Evolution. Photographer of our team. Drew all the fantastic picture of us!

Mu Huanwen

Carried out all the assays involved in Project Evolution and testing the mutant Cas9 in HEK 293 cells

Zhang Junyi

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, preparation of PCR products for deep sequencing.

Shaun Lim Wen Zheng

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, preparation of PCR products for deep sequencing.

Ming Jie

Helped out during our exhibition

Bui Thuy Thien

Helped out during our exhibition

Jenndy

Helped out during our exhibition