Team:British Columbia/Project/Bio-Pathways
Biosynthetic Pathways
Abstract
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Key Achievements
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Introduction
Escherichia coli is a commonly used synthetic biology platform for biosynthesis of valuable chemicals, attractive due to the availability of genetic manipulation tools and ease of growth and scalability. Our project aimed to utilize engineered E.coli to convert the sugars released from the S-layer degradation of cellulose into value-added chemicals, as a means of reducing the cost of starting materials for these valuable chemicals. Past iGEM teams have already built many biosynthesis pathways into E. coli, including the pathway for β-carotene. We used the previously BioBricked pathway for β-carotene as a proof of concept because β-carotene is coloured, making it easily detectable. β-carotene is naturally found in fruits and vegetables, such as carrots. Coupling the E. coli production system for β-carotene with cheap starting materials, such as plant biomass, suggests that high value chemicals with E. coli biosynthesis pathways could also be produced from cheap starting materials
Design
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Methods
Results
Conclusion
References
Check out other parts of our project below!
