Team:ShanghaitechChina/Attributions

igem2016:ShanghaiTech

General

iGem is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.

Logistics: Chen Yifan, Liu Yi

Abstract: Liu Yi, Ba Fang, Gu Shijie

Parts: Gu Shijie; Chen Yifan

Webpage construction and design: Shen Yifei

wiki Big Picture: Bafang, Gu Shijie

wiki design: Shen Yifei

wiki home background: Ye Qinying

wiki home video: Gu Shijie, Zhang Jiaming, Ye Qinying, Qian Lechen.

wiki logo: Ye Qinying

Wiki team avatar: JSNU

Banner&Post: Ba Fang

InterLab

We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.

LiuYi BaFang;Zhu Haolong;Wang Qiaoqi

Hydrogenase Section

Clone Design: Sun Yilin, Chen Yifan, Nie Yan, LiuYi

In this part Nie Yan provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Nie Yan’s Lab, SIAIS. The help of Nie Yan was involved in the the beginning, but the principle is that all of the experiments are done by ourselves.

Sun Yilin provided us with the tutorials of Snapgene and primer design.

Sequencing Confirm: LiuYi, Chen Yifan

The sequencing is provided by Tsingke Sequencing and Jie Li Sequencing

Clone of histag-TEV-hydA-SpyCatcher into pACE:Yang Haonan, Liu Yi, Ba Fang

Clone of histag-TEV-hydA-SpyTag into pACE: Yang Haonan, Liu Yi, Ba Fang

Clone of hydE into pDC: Chen Yifan, Liu Yi, Ba Fang

Clone of hydF into pDK: Ba Fang, Liu Yi

Clone of hydG into pDS: Liu Yi, Ba Fang

Cre recombination & Result Demonstration: Bafang, LiuYi

Hydrogen Assay: LiuYi, Ba Fang, Mi Qixi, Ding Zetao

Mi Qixi is a PI from School of Physical Science and Technology. Ding Zetao is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.

Preparation of PirLC/DH5a/BL21(DE3) chemistry component cell : LiuYi

Preparation of DH5a electronic component cell : Chen Yifan

HydA Induction and Synthesis: Zhu Haolong, Wang Qiaoqi

HydA Purification: Zhu Haolong, Wang Qiaoqi

HydA Assay & WB Demonstration: Zhu Haolong, Wang Qiaoqi

Wiki of Hydrogenase : Liu Yi, Gu Shijie, Ba Fang

Biofilm Section

An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin.

TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.

Clone of his-CsgA-SpyCatcher-his :Gu Shijie, An Bolin (Graduate Instructor/Advisor)

Clone of his-CsgA-SpyCatcher: Gu Shijie

Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Clone of mcherry-SpyTag: Gu Shijie

Clone of CsgA-SpyCatcher-his: Gu Shijie

Clone of CsgA-SpyCatcher: Gu Shijie

Clone of CsgA: Gu Shijie

Sequencing Confirm: Gu Shijie, An Bolin

An Bolin provided help on Geneious. But the actual alignment was done by Gu Shijie

mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University

ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Colony PCR: Gu Shijie, Qian Lechen

Congo Red Assay(plate): Zheng Weitong, Qian Lechen

Congo Red Assay(solution): Qian Lechen,Zheng Weitong,Zhuang Lei

Expression and purification of mcherry-spycatcher: Zhuang Lei, Qian Lechen, Liu Yi, Zhang Jiaming

Expression and purification of mcherry-spytag: Zhuang Lei, An Bolin, Qian Lehchen

Biofilm culture: Qian Lechen, Zhuanglei, Zheng Weitong

Crystal violet assay: Qian Lechen, Zhang Jiaming

TEM sample preparation: Qian Lechen, Zhang Jiaming，Zhuanglei, Wang Xinyu (Graduate Instructor/Advisor)

TEM: Qian Lechen，Zhang Jiaming, An Bolin, Wang Xinyu

Fluorescence Microscopy: Qian Lechen, An Bolin

Quantum dots(QD) binding test: Qian Lechen,Zhang Jiaming

Different QDs supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Zhang Jiaming, Zhuang Lei

Nanorods binding test: Qian Lechen

Nanorods supply for binding: Zhang Jiaming

mcherry-SpyCatcher and CsgA-SpyTag incubation and sample preparation: Qian Lechen

mcherry-SpyTag and CsgA-SpyCatcher incubation and sample preparation: Qian Lechen

Biofilm expression optimization: Qian Lechen

Confocal fluorescence microscope: Qian Lechen,Li Xiaoming(instructor)

biofilm data analysis and result demostration:Qian Lechen

Biofilm microsphere coculture:Qian Lechen

Reagent and material preparation:Zheng Weitong, Qian Lechen, Gu Shijie, Zhang Jiaming, Zhuanglei

notebook data and protocal collection ：Zheng Weitong

wiki of biofilm: Qian Lechen, Gu Shijie

wiki biofilm picture: Qian Lechen

Nano material Section

Pu Jiahua and Wang Xinyu are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and UV-Vis spectrometer characterization. Wang helped in the synthesis of HS-NTA synthesis, TEM characterization and PL spectrometer characterization.

Quantum dots, nanorods and ligand synthesis: Zhang Jiaming, Zhuang Lei, Wang Kang

Quantum dot and nanorods characterization:Zhang Jiaming

wiki of Quantum Dots: Zhang Jiaming, Zhuang lei

Human Practice Section

Team Collaboration with iGEM at Jiangsu Normal University: Yi Liu; Fang Ba; Haolong Zhu

Team Collaboration with iGEM at Tsinghua University: Lechen Qian; Haolong Zhu

Public Questionnaire Design: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang

Public Questionnaire Analysis: Haolong Zhu

Interview with Industrial Community: Haolong Zhu; Yi Liu

Interview with Academic Scientists: Haolong Zhu; Lechen Qian; Yi Liu; Jiaming Zhang; Qiaoqi Wang

Interview with Administrative Authority: Haolong Zhu; Yi Liu; Lechen Qian; Jiaming Zhang

Propagation of iGEM (Opening Day for Concordia International School Shanghai): Haolong Zhu; Shijie Gu; Weitong Zheng; Lechen Qian

Wiki of Human Practice: Haolong Zhu

Composition: Haolong Zhu

Photos: Yi Liu; Fang Ba; Haolong Zhu; Lechen Qian