Team:ShanghaitechChina/Proof
Detailed Proof
Engineered Biofilm Device
A. To allow easy recycling of precious semiconductor nanomaterials, we utilized engineered biofilms to anchor nanomaterials via metal coordination chemistry. Please refer to Engineered Biofilms for details of the successful construction and characterization of engineered biofilms that allow firm binding of nanomaterials. Key data are reproduced below.
First, the simplest design, CsgA-Histag.
1. Congo Red：successful secretion and expression
Fig 1. Fluorescence test of CsgA-His binding with nanomaterials
This assay indicates the success in expression of the self-assembly curli fibers.2. Crystal Violet Assay： quantification test of biofilm
Further, we use crystal violet assay to obtain quantitative data about the relative density of cells and biofilm adhesion to multi-wells cluster dishes.
Fig 2.Crystal violet assay of CsgA-Histag.
This assay indicates the success in expression of the self-assembly curli fibers. This difference between induced strains secreted CsgA-Histag and ΔCsgA manifest a distinct extracellular biofilm production in the modified strain.3.Quantum dots fluorescence test: successful binding test of Histag with nanomaterials (CdSeS/CdSe/ZnS core/shell quantum dots)
After confirming that our parts success in biofilm expression, we are going to test the effect of binding between CsgA-Histag mutant and inorganic nanoparticles. The result was consistent with our anticipation: On the left, CsgA-Histag mutant were induced and QDs are attached with biofilms, thus show bright fluorescence. Therefore, we ensure the stable coordinate bonds between CsgA-Histag mutant and QDs.The picture was snapped by ChemiDoc MP,BioRad, false colored.
Fig 3. Fluorescence test of CsgA-His binding with nanomaterials
4. TEM: visualization of binding test
Since biofilm nanofibers are thin and inconspicuous against the background, we harness CdSe QDs binding to highlight the biofilm area.
Fig 4.Representative TEM images of biotemplated CdS quantum dots on CsgA-His.
Finally, transmission electron microscopy(TEM) visualize the microscopic binding effect of CsgA-Histag fused biofilm with CdS nanorods in comparison with image of pure nanofiber composed by CsgA-Histag and one without inducer. Thus we ultimately confirm the viability of bio-abiotic hybrid system.
Fig 5.Representative TEM images of biotemplated CdS nanorods on CsgA-His.
Second, The complex design with extra function of binding SpyTag-linked enzymes in addition to its nanomaterial-binding through Histag. This is realized with our Part BBa_K2132001 under the promoter of aTc.
1. Congo Red：successful secretion and expression
After CR dye, the figure indicates that the His-CsgA-SpyCatcher-Histag mutant induced by 0.25 μg ml-1 of aTc and cultured for 72h at 30℃ successfully secreted a thin-layer biofilm on the plate which are stained to brown-red color by CR. This assay also proved that the new and challenging construction of appending a large protein onto CsgA subunits will work accurately and effectively.
Fig 5. Congo Red Assay of His-CsgA-SpyCatcher-Histag.
2. Quantum dots fluorescence test: successful binding test of Histag with nanomaterials
Then comes to the next part: we want to check if SpyCatcher protein will be too large to cause steric hindrance effect on Histag peptide. The best approach to verify is the fluorescence assay of binding with nanomaterials. We use His-CsgA-SpyCatcher-Histag as demo.
Fig 6. Fluoresence Nanomaterials Binding Assay of His-CsgA-SpyCatcher-Histag
3. TEM: visualization of binding test
TEM further characterize the biofilm expressed by strains secreted His-CsgA-SpyCatcher-Histag (HSCH). The distinct nanofiber network manifests the large biofilm expression.
Fig 7. aTc induced secretion of His-CsgA-SpyCatcher-Histag visualized by TEM. Without the presence of inducer, there’s no nanofiber formation around scattered bacteria.
CsgA-His can interface with different inorganic materials since they form the coordinate bonds with the same ligand, Co-NTA, on nanomaterials. Here we use to AuNPs in place of quantum dots and nanomaterials to characterize the validity of Histags on CsgA fused amyloid protein and meanwhile prove the versatility of our biofilm-based platform. As the figures shown, we confirm the feasibility of our newly constructed biobricks to template inorganic material and thus form bio-abiotic hybrid system.
Fig 8. After aTc induced, biofilm secreted by His-CsgA-SpyCatcher-Histag organizes AuNP around the cells.
5.Spy System binding test: successful functional test of SpyCatcher on CsgA fused protein
In the following part, we try to test the viability of SpyCatcher protein fused on CsgA amyloid subunits to see if it’s ideal to bind with SpyTag on Hydrogenase. As figure illustrated, his-CsgA-SpyCatcher-his mutant incubated with mcherry-SpyTag show a clear biofilm-associated mcherry fluorescence signal, which indicating the accurate conformation and function of the SpyTag and SpyCatcher linkage system. The distinct localization highlight of red fluorescence on E.coli, which to a large extent prove the specificity of our desired linkage between SpyTag and SpyCatcher system.
Fig 9.mcherry-SpyTag fluorescence protein binding test of His-CsgA-SpyCatcher-(Histag).
6.Inducer concentration optimization
We cultured all E.coli mutants in multi-wells with increasing inducer gradient. The result demonstrated in accordance that 0.25 μg ml-1 of aTc will induce the best expression performance of biofilm, which is exactly the inducer concentration we applied in the project.
Fig 10.Inducer concentration gradient test.
Hydrogenese gene clusters
B. Finally, high-activity hydrogenase is necessary for our system. To achieve efficient enzymatic activities, we codon-optimized and constructed the whole hydrogenase gene clusters (from Clostridium Acetobutylicum) by leveraging the multi-expression Acembl System. Please refer to Hydrogenase Session for more details.
