Team:Stony Brook/Notebook
Document the dates you worked on your project.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes:
Week 1 (6/27 - 7/1)
6/27
Cancer Group
- PCR of 3xHA-TDGF1 construct
- Gel of PCR Product → PCR was a failure
- Second PCR of 3xHA-TDGF1 construct → left overnight
Interlab Study
- Measured LUDOX and water for plate reader
- Transformation of positive and negative controls, test devices 1-3
6/28
Cancer Group
- Ran gel on PCR of yesterday's construct → Gel worked
- Gel purification of PCR Product → low concentration of DNA found during nanodrop
- PCR the construct using a revised method
- 2:54pm → ran the gel on the PCR product
Interab Study
- Heat and "SB" streaking seems not to have worked
- No visible red colonies on plate
6/29
Cancer Group
- PCR Not working well → keep trying new methods with different annealing temperatures
- Tried 65 and 67 degrees C
Interlab Study
- FITC started
- Cutting re-inoculated
- Ordered new LUDOX
Other
- College of Arts and Sciences Pre College Summer Institute students stopped by lab
- Spoke to them about information on synthetic biology and iGEM
6/30
Cancer Group
- PCR worked → Test 5 → changed annealing temperature to 66 degrees C + increased the denaturing time
- Nanodropped DNA
7/1
Cancer Group
- Digestion of TDGF-1 construct and YFP352GAP vector
TDGF1 Construct
