Team:Stony Brook/Notebook/Cancer-W6

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Week 4: 8/1-8/5

8/1

Emmanuel Ligation (193 ng/ul)
    Content Experimental (ul) Control (ul)
    H2O 38.8 39.8
    Cutsmart 5.0 5.0
    XbaI 0.5 X
    EcoRI-HF 0.5 X
    DNA 5.18 5.18

Gel run

Lanes:

  1. Ladder
  2. Emmanuel Digest Control
  3. Emmanuel Digest Experimental
  • Did not work

25 ul Digest of Ryan + Emmanuel (Veronica) Miniprep
  • Made 17 LB With Ampicillin (600ml)
Ryan - 446.4 ng/ul
    Content Experimental (ul) Control (ul)
    H2O 20.4 21.4
    Cutsmart 2.5 2.5
    XbaI 0.5 X
    EcoRI-HF 0.5 X
    DNA 1.12 1.12
Emmanuel - 193 ng/ul
    Content Experimental (ul) Control (ul)
    H2O 18.9 19.9
    Cutsmart 2.5 2.5
    XbaI 0.5 X
    EcoRI-HF 0.5 X
    DNA 2.59 2.59

8/2

Miniprep
  • Miniprep of ligated construct
  • 10,000rpm for 3 minutes at 25°C
Ran a nanodrop of Minipreps
    Content Concentration (ng/ul) 260/280
    Veronica 1 311.5 1.86
    Veronica 2 43.8 1.76
    Veronica 3 259 1.88
    Veronica 4 296 1.88
    R1 176.5 1.75
    Ryan 2 277.3 1.85
    Ryan 3 301.6 1.81
    Ryan 4 366.6 1.82
Ran a gel of miniprepped plasmids
  • Used 5ul of DNA and 1 ul of dye

Lanes:

  1. Veronica 1
  2. Veronica 3
  3. Veronica 4
  4. Ladder
  5. Ryan 1
  6. Ryan 2
  7. Ryan 3
  8. Ryan 4