Team:Stony Brook/Notebook/Cancer-W7

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Week 7: 8/8 - 8/14

Week 7: 8/8 - 8/14

8/8

PCR Table Template:
    Contents ul
    Phire 25
    H2O 23.2
    50 uM F Primer 0.5
    50 uM R Primer 0.5
    95.1ug/ul Construct 0.8

PCR Settings Template:

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 60
    Denaturation 98 25
    Annealing 66 20
    Extension 72 45
    Final Extension 72 60

Nanodrop Template:

    Content Concentration (ng/ul) 260/280
    Construct 14.6 1.85
    Vector 14.6 1.80

Ligation Template

    Content ul
    10X T4 Buffer 2
    Vector 4 (58.4 ng)
    Construct 1.55 (22.69 ng)
    H2O 11.45
    Ligase 1

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