Team:Stony Brook/Notebook/Cancer-W7

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Week 7: 8/8 - 8/14

I left you some of the commonly used templates down there under 8/8. Just fill them in as needed. I'll only really have wifi for a few hours on two days between Saturday and Thursday. If you need anything, message me on Facebook and I'll get back to you as soon as I can.



J OSHA

Week 7: 8/8 - 8/14

8/8

PCR Table Template:
    Contents ul
    Phire 25
    H2O 23.2
    50 uM F Primer 0.5
    50 uM R Primer 0.5
    95.1ug/ul Construct 0.8

PCR Settings Template:

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 60
    Denaturation 98 25
    Annealing 66 20
    Extension 72 45
    Final Extension 72 60

Nanodrop Template:

    Content Concentration (ng/ul) 260/280
    Construct 14.6 1.85
    Vector 14.6 1.80

Ligation Template

    Content ul
    10X T4 Buffer 2
    Vector 4 (58.4 ng)
    Construct 1.55 (22.69 ng)
    H2O 11.45
    Ligase 1

Multiple Digests Template:

Lane 1: PIggyBac - 121.2 ng/ul
    Content Experimental ul Control ul
    H2O 35.75 36.75
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 8.25 8.25
Lane 2: PIggyBac 1:10 - 423.7 ng/ul
    Content Experimental ul Control ul
    H2O 41,6 42.6
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 2.36 2.36

Lane 4: Ryan - 446.4 ng/ul
    Content Experimental ul Control ul
    H2O 41.8 42.8
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 2.24 2.24
Lane 7: Ryan 3 - 272.6 ng/ul
    Content Experimental ul Control ul
    H2O 40.3 41.3
    Cutsmart 5 5
    EcoRI-HF 0.5 X
    XbaI 0.5 X
    DNA 3.67 3.67

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8/14