I left you some of the commonly used templates down there under 8/8. Just fill them in as needed. I'll only really have wifi for a few hours on two days between Saturday and Thursday. If you need anything, message me on Facebook and I'll get back to you as soon as I can.
With the warmest love and regards
J OSHUA
8/8
PCR Table Template:
| Contents |
ul |
Phire |
25 |
| H2O |
23.2 |
| 50 uM F Primer |
0.5 |
| 50 uM R Primer |
0.5 |
| 95.1ug/ul Construct |
0.8 |
PCR Settings Template:
| Phase |
Temperature (°C) |
Time (sec) |
| Initial Denaturation |
98 |
60 |
| Denaturation |
98 |
25 |
| Annealing |
66 |
20 |
| Extension |
72 |
45 |
| Final Extension |
72 |
60 |
Nanodrop Template:
| Content |
Concentration (ng/ul) |
260/280 |
| Construct |
14.6 |
1.85 |
| Vector |
14.6 |
1.80 |
Ligation Template
| Content |
ul |
| 10X T4 Buffer |
2 |
| Vector |
4 (58.4 ng) |
Construct |
1.55 (22.69 ng) |
| H2O |
11.45 |
| Ligase |
1 |
Multiple Digests Template:
Lane 1: PIggyBac - 121.2 ng/ul
| Content |
Experimental ul |
Control ul |
| H2O |
35.75 |
36.75 |
| Cutsmart |
5 |
5 |
| EcoRI-HF |
0.5 |
X |
| XbaI |
0.5 |
X |
| DNA |
8.25 |
8.25 |
Lane 2: PIggyBac 1:10 - 423.7 ng/ul
| Content |
Experimental ul |
Control ul |
| H2O |
41,6 |
42.6 |
| Cutsmart |
5 |
5 |
| EcoRI-HF |
0.5 |
X |
| XbaI |
0.5 |
X |
| DNA |
2.36 |
2.36 |
Lane 4: Ryan - 446.4 ng/ul
| Content |
Experimental ul |
Control ul |
| H2O |
41.8 |
42.8 |
| Cutsmart |
5 |
5 |
| EcoRI-HF |
0.5 |
X |
| XbaI |
0.5 |
X |
| DNA |
2.24 |
2.24 |
Lane 7: Ryan 3 - 272.6 ng/ul
| Content |
Experimental ul |
Control ul |
| H2O |
40.3 |
41.3 |
| Cutsmart |
5 |
5 |
| EcoRI-HF |
0.5 |
X |
| XbaI |
0.5 |
X |
| DNA |
3.67 |
3.67 |
