Team:Stony Brook/Notebook/Cancer-W9

• People
  Team Attributions Sponsors
• Community Involvement
  Engagement and Outreach Human Practices Collaborations Safety
• Project
  Description Protocols Parts Notebook Interlab Study
• Home

Week 9: 8/22 - 8/28

---

---

8/22

PCR of new IDT Shipments

AmilCP

Contents	ul
Q5 MasterMix	12.5
H2O	9
10 uM F Primer	1.25
10 uM R Primer	1.25
Template	1

IDT #1

Contents	ul
Q5 MasterMix	12.5
H2O	6.5
10 uM F Primer	2.5
10 uM R Primer	2.5
Template	1

IDT #2

Contents	ul
Q5 MasterMix	12.5
H2O	6.5
10 uM F Primer	2.5
10 uM R Primer	2.5
Template	1

PCR Settings: 25 Cycles

Phase	Temperature (°C)	Time (sec)
Initial Denaturation	98	30
Denaturation	98	10
Annealing	65	30
Extension	72	120
Final Extension	72	120

---

8/23

---

8/24

---