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<div class="sec page_title"> | <div class="sec page_title"> | ||
− | + | <div> | |
− | + | <h1>BASIC PARTS</h1> | |
− | + | </div> | |
</div> | </div> | ||
<div class="sec white"> | <div class="sec white"> | ||
− | + | <div> | |
− | + | <table> | |
− | + | <th>Part Name</th> | |
− | + | <th>Biobrick</th> | |
− | + | <th>Comments</th> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Transcriptional regulator esaR</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116001">BBa_K2116001</a></td> | |
− | + | <td>Transcriptional repressor, binds esaboxes. </td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>mNectarine</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116016">BBa_K2116016</a></td> | |
− | + | <td>Red fluorescent protein mNectarine.</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>mNectarine+ssrA tag</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116018">BBa_K2116018</a></td> | |
− | + | <td>Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag </td> | |
− | + | </tr> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>bxb1 integrase+ssrA</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K21160009">BBa_K2116009</a></td> | |
− | + | <td>bxb1 integrase with a fast ssrA degradation tag </td> | |
− | + | </tr> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>tp901 integrase+ssrA</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116010">BBa_K2116010</a></td> | |
− | + | <td>Red fluorescent protein mNectarine with a moderately fast ssrA tag </td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pNorV</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">BBa_K2116002</a></td> | |
− | + | <td>Nitric Oxide responsive promoter</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>superfolder GFP</td> | |
− | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116017">BBa_K2116017</a></td> | |
− | + | <td>Fast maturing and stable green fluorescent protein </td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>NO and AHL responsive AND gate</td> | |
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116011">BBa_K2116011</a></td> | ||
+ | <td>This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and | ||
+ | nitric oxide for transcription. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>bxb1 integrase</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116026">BBa_K2116026</a></td> | ||
+ | <td>bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>tp901 integrase</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116035">BBa_K2116035</a></td> | ||
+ | <td>tp901 integrase, can flip any sequence flanked by its attP and attB binding sites. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EsaR regulated promoter</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116025">BBa_K2116025</a></td> | ||
+ | <td>The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiateion only in presence of 3OC6HSL. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EsaR regulated promoter</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116066">BBa_K2116066</a></td> | ||
+ | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiateion only in presence of 3OC6HSL. </td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
Revision as of 22:28, 30 November 2016
BASIC PARTS
Part Name | Biobrick | Comments |
---|---|---|
Transcriptional regulator esaR | BBa_K2116001 | Transcriptional repressor, binds esaboxes. |
mNectarine | BBa_K2116016 | Red fluorescent protein mNectarine. |
mNectarine+ssrA tag | BBa_K2116018 | Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag |
bxb1 integrase+ssrA | BBa_K2116009 | bxb1 integrase with a fast ssrA degradation tag |
tp901 integrase+ssrA | BBa_K2116010 | Red fluorescent protein mNectarine with a moderately fast ssrA tag |
pNorV | BBa_K2116002 | Nitric Oxide responsive promoter |
superfolder GFP | BBa_K2116017 | Fast maturing and stable green fluorescent protein |
NO and AHL responsive AND gate | BBa_K2116011 | This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription. |
bxb1 integrase | BBa_K2116026 | bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites. |
tp901 integrase | BBa_K2116035 | tp901 integrase, can flip any sequence flanked by its attP and attB binding sites. |
EsaR regulated promoter | BBa_K2116025 | The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiateion only in presence of 3OC6HSL. |
EsaR regulated promoter | BBa_K2116066 | The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiateion only in presence of 3OC6HSL. |