Line 73: | Line 73: | ||
<td>EsaR regulated promoter</td> | <td>EsaR regulated promoter</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116025">BBa_K2116025</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116025">BBa_K2116025</a></td> | ||
− | <td>The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription | + | <td>The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiation only |
+ | in presence of f <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> | ||
</tr> | </tr> | ||
− | + | <tr> | |
<td>EsaR regulated promoter</td> | <td>EsaR regulated promoter</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116066">BBa_K2116066</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116066">BBa_K2116066</a></td> | ||
− | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription | + | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription |
+ | initiation only in presence of f <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EsaR regulated promoter</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116037">BBa_K2116037</a></td> | ||
+ | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 15bp spacer. Transcription | ||
+ | initiation only in presence of f <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Lactate regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116027">BBa_K2116027</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Lactate regulated promoter</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116049">BBa_K2116049</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116004">BBa_K2116004</a></td> | ||
+ | <td>PnorV promoter with its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL f <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116005">BBa_K2116005</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116006">BBa_K2116006</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116012">BBa_K2116012</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116013">BBa_K2116013</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116067">BBa_K2116067</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116007">BBa_K2116007</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116008">BBa_K2116008</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116068">BBa_K2116068</a></td> | ||
+ | <td>?</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>NO and AHL regulated AND gate</td> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116069">BBa_K2116069</a></td> | ||
+ | <td>?</td> | ||
</tr> | </tr> | ||
Revision as of 08:47, 1 December 2016
BASIC PARTS
Part Name | Biobrick | Comments |
---|---|---|
Transcriptional regulator esaR | BBa_K2116001 | Transcriptional repressor, binds esaboxes. |
mNectarine | BBa_K2116016 | Red fluorescent protein mNectarine. |
mNectarine+ssrA tag | BBa_K2116018 | Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag |
bxb1 integrase+ssrA | BBa_K2116009 | bxb1 integrase with a fast ssrA degradation tag |
tp901 integrase+ssrA | BBa_K2116010 | Red fluorescent protein mNectarine with a moderately fast ssrA tag |
pNorV | BBa_K2116002 | Nitric Oxide responsive promoter |
superfolder GFP | BBa_K2116017 | Fast maturing and stable green fluorescent protein |
NO and AHL responsive AND gate | BBa_K2116011 | This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription. |
bxb1 integrase | BBa_K2116026 | bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites. |
tp901 integrase | BBa_K2116035 | tp901 integrase, can flip any sequence flanked by its attP and attB binding sites. |
EsaR regulated promoter | BBa_K2116025 | The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiation only in presence of f 3OC6HSL. |
EsaR regulated promoter | BBa_K2116066 | The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiation only in presence of f 3OC6HSL. |
EsaR regulated promoter | BBa_K2116037 | The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 15bp spacer. Transcription initiation only in presence of f 3OC6HSL. |
Lactate regulated AND gate | BBa_K2116027 | ? |
Lactate regulated promoter | BBa_K2116049 | ? |
NO and AHL regulated AND gate | BBa_K2116004 | PnorV promoter with its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL f 3OC6HSL. |
NO and AHL regulated AND gate | BBa_K2116005 | ? |
NO and AHL regulated AND gate | BBa_K2116006 | ? |
NO and AHL regulated AND gate | BBa_K2116012 | ? |
NO and AHL regulated AND gate | BBa_K2116013 | ? |
NO and AHL regulated AND gate | BBa_K2116067 | ? |
NO and AHL regulated AND gate | BBa_K2116007 | ? |
NO and AHL regulated AND gate | BBa_K2116008 | ? |
NO and AHL regulated AND gate | BBa_K2116068 | ? |
NO and AHL regulated AND gate | BBa_K2116069 | ? |