Difference between revisions of "Team:ETH Zurich/Basic Part"
| Line 28: | Line 28: | ||
<td>mNectarine+ssrA tag</td> | <td>mNectarine+ssrA tag</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116018">BBa_K2116018</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116018">BBa_K2116018</a></td> | ||
| − | <td>Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag </td> | + | <td>Red fluorescent protein mNectarine with a <b>moderately fast</b> ssrA degradation tag </td> |
</tr> | </tr> | ||
</tr> | </tr> | ||
| Line 34: | Line 34: | ||
<td>bxb1 integrase+ssrA</td> | <td>bxb1 integrase+ssrA</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K21160009">BBa_K2116009</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K21160009">BBa_K2116009</a></td> | ||
| − | <td>bxb1 integrase with a fast ssrA degradation tag </td> | + | <td>bxb1 integrase with a <b>fast</b> ssrA degradation tag </td> |
</tr> | </tr> | ||
</tr> | </tr> | ||
| Line 40: | Line 40: | ||
<td>tp901 integrase+ssrA</td> | <td>tp901 integrase+ssrA</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116010">BBa_K2116010</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116010">BBa_K2116010</a></td> | ||
| − | <td>Red fluorescent protein mNectarine with a moderately fast ssrA tag </td> | + | <td>Red fluorescent protein mNectarine with a <b>moderately fast</b> ssrA tag </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>pNorV</td> | <td>pNorV</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">BBa_K2116002</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116002">BBa_K2116002</a></td> | ||
| − | <td>Nitric Oxide responsive promoter</td> | + | <td><b>Nitric Oxide</b> responsive promoter</td> |
</tr> | </tr> | ||
| Line 57: | Line 57: | ||
<td>NO and AHL responsive AND gate</td> | <td>NO and AHL responsive AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116011">BBa_K2116011</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116011">BBa_K2116011</a></td> | ||
| − | <td>This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and | + | <td>This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a> and |
nitric oxide for transcription. </td> | nitric oxide for transcription. </td> | ||
</tr> | </tr> | ||
| Line 73: | Line 73: | ||
<td>EsaR regulated promoter</td> | <td>EsaR regulated promoter</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116025">BBa_K2116025</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116025">BBa_K2116025</a></td> | ||
| − | <td>The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiation only | + | <td>The Bba_J23118 promoter with an additional <b>esabox</b> downstream of transcription start site. Transcription initiation only |
| − | in presence of | + | in presence of <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>EsaR regulated promoter</td> | <td>EsaR regulated promoter</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116066">BBa_K2116066</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116066">BBa_K2116066</a></td> | ||
| − | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription | + | <td>The Bba_J23118 promoter with <b>two esaboxes</b> downstream of transcription start site, separated by an <b>8bp spacer</b>. Transcription |
| − | initiation only in presence of | + | initiation only in presence of <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>EsaR regulated promoter</td> | <td>EsaR regulated promoter</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116037">BBa_K2116037</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116037">BBa_K2116037</a></td> | ||
| − | <td>The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 15bp spacer. Transcription | + | <td>The Bba_J23118 promoter with <b>two esaboxes</b< downstream of transcription start site, separated by an <b>15bp spacer</b>. Transcription |
| − | initiation only in presence of | + | initiation only in presence of <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 101: | Line 101: | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116004">BBa_K2116004</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116004">BBa_K2116004</a></td> | ||
| − | <td>PnorV promoter with its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL | + | <td>PnorV promoter <b>with</b> its native spacer after transcription start site, followed by <b>one esabox</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116005">BBa_K2116005</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116005">BBa_K2116005</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>with</b> its native spacer after transcription start site, followed by <b>two esaboxes 8bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116006">BBa_K2116006</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116006">BBa_K2116006</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>with</b> its native spacer after transcription start site, followed by <b>three esaboxes 8bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116012">BBa_K2116012</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116012">BBa_K2116012</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>without</b> its native spacer after transcription start site, followed by <b>one esabox</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116013">BBa_K2116013</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116013">BBa_K2116013</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>without</b> its native spacer after transcription start site, followed by <b>two esaboxes 8bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116067">BBa_K2116067</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116067">BBa_K2116067</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>without</b> its native spacer after transcription start site, followed by <b>three esaboxes 8bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116007">BBa_K2116007</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116007">BBa_K2116007</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>with</b> its native spacer after transcription start site, followed by <b>two esaboxes 15bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116008">BBa_K2116008</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116008">BBa_K2116008</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>with</b> its native spacer after transcription start site, followed by <b>three esaboxes 15bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116068">BBa_K2116068</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116068">BBa_K2116068</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>without</b> its native spacer after transcription start site, followed by <b>two esaboxes 15bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>NO and AHL regulated AND gate</td> | <td>NO and AHL regulated AND gate</td> | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116069">BBa_K2116069</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2116069">BBa_K2116069</a></td> | ||
| − | <td> | + | <td>PnorV promoter <b>without</b> its native spacer after transcription start site, followed by <b>three esaboxes 15bp apart</b>. This AND gate is active in presence of nitric oxide and the AHL <a href="http://parts.igem.org/3OC6HSL">3OC6HSL</a>.</td> |
</tr> | </tr> | ||
Revision as of 09:00, 1 December 2016
BASIC PARTS
| Part Name | Biobrick | Comments |
|---|---|---|
| Transcriptional regulator esaR | BBa_K2116001 | Transcriptional repressor, binds esaboxes. |
| mNectarine | BBa_K2116016 | Red fluorescent protein mNectarine. |
| mNectarine+ssrA tag | BBa_K2116018 | Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag |
| bxb1 integrase+ssrA | BBa_K2116009 | bxb1 integrase with a fast ssrA degradation tag |
| tp901 integrase+ssrA | BBa_K2116010 | Red fluorescent protein mNectarine with a moderately fast ssrA tag |
| pNorV | BBa_K2116002 | Nitric Oxide responsive promoter |
| superfolder GFP | BBa_K2116017 | Fast maturing and stable green fluorescent protein |
| NO and AHL responsive AND gate | BBa_K2116011 | This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription. |
| bxb1 integrase | BBa_K2116026 | bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites. |
| tp901 integrase | BBa_K2116035 | tp901 integrase, can flip any sequence flanked by its attP and attB binding sites. |
| EsaR regulated promoter | BBa_K2116025 | The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiation only in presence of 3OC6HSL. |
| EsaR regulated promoter | BBa_K2116066 | The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiation only in presence of 3OC6HSL. |
| EsaR regulated promoter | BBa_K2116037 | The Bba_J23118 promoter with two esaboxes15bp spacer. Transcription initiation only in presence of 3OC6HSL. |
| Lactate regulated AND gate | BBa_K2116027 | ? |
| Lactate regulated promoter | BBa_K2116049 | ? |
| NO and AHL regulated AND gate | BBa_K2116004 | PnorV promoter with its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116005 | PnorV promoter with its native spacer after transcription start site, followed by two esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116006 | PnorV promoter with its native spacer after transcription start site, followed by three esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116012 | PnorV promoter without its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116013 | PnorV promoter without its native spacer after transcription start site, followed by two esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116067 | PnorV promoter without its native spacer after transcription start site, followed by three esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116007 | PnorV promoter with its native spacer after transcription start site, followed by two esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116008 | PnorV promoter with its native spacer after transcription start site, followed by three esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116068 | PnorV promoter without its native spacer after transcription start site, followed by two esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
| NO and AHL regulated AND gate | BBa_K2116069 | PnorV promoter without its native spacer after transcription start site, followed by three esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL. |
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