BASIC PARTS
Part Name | Biobrick | Comments |
---|---|---|
Transcriptional regulator esaR | BBa_K2116001 | Transcriptional repressor, binds esaboxes. |
mNectarine | BBa_K2116016 | Red fluorescent protein mNectarine. |
mNectarine+ssrA tag | BBa_K2116018 | Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag |
bxb1 integrase+ssrA | BBa_K2116009 | bxb1 integrase with a fast ssrA degradation tag |
tp901 integrase+ssrA | BBa_K2116010 | Red fluorescent protein mNectarine with a moderately fast ssrA tag |
pNorV | BBa_K2116002 | Nitric Oxide responsive promoter |
superfolder GFP | BBa_K2116017 | Fast maturing and stable green fluorescent protein |
NO and AHL responsive AND gate | BBa_K2116011 | This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription. |
bxb1 integrase | BBa_K2116026 | bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites. |
tp901 integrase | BBa_K2116035 | tp901 integrase, can flip any sequence flanked by its attP and attB binding sites. |
EsaR regulated promoter | BBa_K2116025 | The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiateion only in presence of 3OC6HSL. |
EsaR regulated promoter | BBa_K2116066 | The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiateion only in presence of 3OC6HSL. |