Team:ETH Zurich/Basic Part

BASIC PARTS

Part Name Biobrick Comments
Transcriptional regulator esaR BBa_K2116001 Transcriptional repressor, binds esaboxes.
mNectarine BBa_K2116016 Red fluorescent protein mNectarine.
mNectarine+ssrA tag BBa_K2116018 Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag
bxb1 integrase+ssrA BBa_K2116009 bxb1 integrase with a fast ssrA degradation tag
tp901 integrase+ssrA BBa_K2116010 Red fluorescent protein mNectarine with a moderately fast ssrA tag
pNorV BBa_K2116002 Nitric Oxide responsive promoter
superfolder GFP BBa_K2116017 Fast maturing and stable green fluorescent protein
NO and AHL responsive AND gate BBa_K2116011 This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription.
bxb1 integrase BBa_K2116026 bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites.
tp901 integrase BBa_K2116035 tp901 integrase, can flip any sequence flanked by its attP and attB binding sites.
EsaR regulated promoter BBa_K2116025 The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiateion only in presence of 3OC6HSL.
EsaR regulated promoter BBa_K2116066 The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiateion only in presence of 3OC6HSL.

Thanks to the sponsors that supported our project: